Antimicrobial and antitumor activity and diversity of endophytic fungi from traditional Chinese medicinal plant Cephalotaxus hainanensis Li
| Autor: | Lin St, Wu Li, Sixin Liu, Cao Xy, Liu Yh, Xiaoping Hu, Xu Cb, Congfa Li, H.R. Yang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fusarium 030106 microbiology Antineoplastic Agents HL-60 Cells Cephalotaxus hainanensis Microbial Sensitivity Tests Plant use of endophytic fungi in defense Cephalotaxus 03 medical and health sciences Anti-Infective Agents Cell Line Tumor Fusarium oxysporum Colletotrichum Endophytes Genetics Humans Medicine Chinese Traditional Molecular Biology Biological Products biology Traditional medicine food and beverages Hep G2 Cells General Medicine Nemania diffusa biology.organism_classification Antimicrobial Plant Leaves K562 Cells |
| Zdroj: | Genetics and Molecular Research. 15 |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/gmr.15028633 |
| Popis: | Endophytes from Cephalotaxus hainanensis Li, an important source of anti-leukemia drugs, have not been widely explored. In this study, 265 endophytic fungal isolates from C. hainanensis Li were screened for antimicrobial activities against tilapia, banana, rice, and rape and for antitumor activities against human leukemia cell lines (K562, NB4, and HL-60). Diversity was also analyzed. The results showed that 17.7% of the endophytic fungi had antimicrobial activities against at least three different test microbes, and activity against Fusarium oxysporum RKY102 was the highest at 15.8%. Cytotoxicity against at least one tumor cell line tested was observed in 18.5% of the endophytic fungi; with the highest value of 10.6% against K562. The endophytic fungal strains also showed relatively high activities against K562, NB4, and HL-60 while relatively fewer strains were cytotoxic against the human hepatic Hep-G2 and colon LoVo cancer cell lines. Thirty endophytic fungal strains showed both high antimicrobial and antitumor activities. Moreover, the analyses of the diversity of the 30 highly active strains showed they belonged to 20 species from 14 genera, and this is the first report of endophytic fungi Albonectria rigidiuscula, Colletotrichum magnisporum, and Nemania diffusa being isolated from Cephalotaxus plants. These findings suggest that natural antibacterial products for humans and tilapia; antifungal compounds for rice, rape, and banana; and antitumor compounds for leukemia therapy could be isolated from fungal strains derived from C. hainanensis Li. |
| Databáze: | OpenAIRE |
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