Mass spectrometry-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from follicular fluid
| Autor: | Iris Car, Tamara Tramišak Milaković, Sanja Dević Pavlić, Anđelka Radojčić Badovinac, Sandra Kraljević Pavelić, Marko Klobučar, Neda Smiljan Severinski |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Infertility Adult Proteomics endocrine system Glycosylation Proteome QH301-705.5 medicine.medical_treatment Stimulation Fertilization in Vitro Biology General Biochemistry Genetics and Molecular Biology Mass Spectrometry Andrology 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Human fertilization Ovulation Induction In vitro fertilization medicine Humans Biology (General) Glycomics glycoproteins follicular fluid glycome maldi mass spectrometry 030219 obstetrics & reproductive medicine In vitro fertilisation BIOTEHNIČKE ZNANOSTI. Biotehnologija General Medicine inflammation MALDI mass spectrometry medicine.disease Oocyte Follicular fluid BIOTECHNICAL SCIENCES. Biotechnology Follicular Fluid 030104 developmental biology medicine.anatomical_structure chemistry Immunoglobulin G Female in vitro fertilization |
| Zdroj: | Biomolecular Concepts, Vol 11, Iss 1, Pp 153-171 (2020) Biomolecular Concepts Volume 11 Issue 1 |
| ISSN: | 1868-503X |
| Popis: | Couples with infertility issues have been assisted by in vitro fertilization reproduction technologies with high success rates of 50-80%. However, complications associated with ovarian stimulation remain, such as ovarian hyperstimulation. Oocyte quality is a significant factor impacting the outcome of in vitro fertilization procedures, but other processes are also critical for fertilization success. Increasing evidence points to aberrant inflammation as one of these critical processes reflected in molecular changes, including glycosylation of proteins. Here we report results from a MALDI-TOF-MS-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from the follicular fluid obtained from patients undergoing fertilization through either (1) assisted reproduction by modified natural cycle or (2) controlled ovarian stimulation (GnRH antagonist, GnRH Ant) protocols. Significant inflammatory-related differences between analyzed N-glycomes were observed from samples and correlated with the ovarian stimulation protocol used in patients. |
| Databáze: | OpenAIRE |
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