Effects of resistance training and estrogen replacement on adipose tissue inflammation in ovariectomized rats

Autor: Fabiano Candido Ferreira, Marina Rodrigues Barbosa, Sabrina Messa Peviani, Mateus Moraes Domingos, Markus Vinicius Campos Souza, Fábio Santos Lira, Natália Santanielo Silva-Magosso, Gustavo Henrique Rigo Canevazzi, Uliana Sbeguen Stotzer, Sérgio Eduardo de Andrade Perez, Heloisa Sobreiro Selistre de Araújo, Maria Fernanda Cury Rodrigues
Přispěvatelé: Universidade Federal de São Carlos (UFSCar), Universidade Federal de Sergipe (UFS), Universidade Estadual Paulista (Unesp)
Rok vydání: 2017
Předmět:
0301 basic medicine
Blood Glucose
medicine.medical_specialty
Physiology
medicine.drug_class
Endocrinology
Diabetes and Metabolism
Ovariectomy
Adipose tissue
030209 endocrinology & metabolism
Inflammation
Body Mass Index
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Physiology (medical)
Internal medicine
Physical Conditioning
Animal
Gene expression
medicine
Adipocytes
Animals
Nutrition and Dietetics
Estradiol
business.industry
Tumor Necrosis Factor-alpha
Estrogen Replacement Therapy
General Medicine
Silastic
Estrogen
Reverse transcriptase
Resistance training
Interleukin-10
Rats
030104 developmental biology
Endocrinology
Adipose Tissue
Ovariectomized rat
Cytokines
Female
Adiponectin
medicine.symptom
business
hormones
hormone substitutes
and hormone antagonists
Hormone
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1715-5320
Popis: Made available in DSpace on 2018-12-11T16:47:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Estrogen deficiency is directly related to central obesity and low-grade inflammation. Hormonal replacement and exercise training are both able to decrease fat accumulation and inflammation in postmenopausal women. However, the efficiency of resistance training (RT) and estrogen replacement (ER) in minimizing adiposity and inflammation in the visceral adipose tissue (VAT) of ovariectomized (OVX) rats has not yet been elucidated. In this study, Sprague–Dawley rats were divided into the following 6 groups: sham-operated sedentary (Sham-Sed), OVX-Sed, Sham-RT, OVX-RT, OVX-Sed-ER, and OVX-RT-ER groups. ER was performed by implanting silastic capsules containing 17β-estradiol. For RT, the animals were required to climb a 1.1-m vertical ladder with conical flasks containing weights attached to their tails for 12 weeks. Histological analyses were used to evaluate morphological changes. Gene expression levels were determined by quantitative real-time reverse transcriptase polymerase chain reaction, and protein concentrations were determined using Multiplex/Luminex assays. Ovariectomy increased the body mass (BM), adipocyte area, and inflammation in the VAT, the latter of which was indicated by reduced interleukin-10 (48%) and increased tumor necrosis factor (TNF)-α concentration (~3%). RT efficiently decreased BM, adipocyte area, and inflammation in the OVX groups. The combination of RT and ER decreased BM (19%) and the TNF-α concentration (18%) and increased the gene and protein expression levels of adiponectin (173% and 18%). These results indicate that RT and the combination of RT and ER are efficient strategies for reducing the BM and improving the inflammatory status of OVX rats. Department of Physiological Sciences Federal University of São Carlos UFSCar Department of Health Education Federal University of Sergipe Exercise and Immunometabolism Research Group Department of Physical Education University State Estadual Paulista UNESP Exercise and Immunometabolism Research Group Department of Physical Education University State Estadual Paulista UNESP FAPESP: 00798-2
Databáze: OpenAIRE
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