Citrate-capped silver nanoparticles as a probe for sensitive and selective colorimetric and spectrophotometric sensing of creatinine in human urine
| Autor: | Leshern Karamchand, Jonathan M. Blackburn, Melisew Tadele Alula, Nicolette R. Hendricks |
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| Rok vydání: | 2018 |
| Předmět: |
Silver
Surface Properties Metal Nanoparticles Renal function 02 engineering and technology 01 natural sciences Biochemistry Citric Acid Silver nanoparticle Analytical Chemistry Colloid chemistry.chemical_compound Humans Environmental Chemistry Particle Size Surface plasmon resonance Spectroscopy Fluorescent Dyes Detection limit Creatinine Chromatography Aqueous solution Chemistry 010401 analytical chemistry Hydrogen-Ion Concentration Surface Plasmon Resonance 021001 nanoscience & nanotechnology 0104 chemical sciences Colorimetry Spectrophotometry Ultraviolet Particle size 0210 nano-technology |
| Zdroj: | Analytica Chimica Acta. 1007:40-49 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2017.12.016 |
| Popis: | Urinary creatinine concentration is a critical physiological parameter that enables reliable assessment of patient renal function and diagnosis of a broad spectrum of diseases. In this study, a simple and inexpensive sensor comprising monodisperse, citrate-capped silver nanoparticles (cc-AgNPs) was developed, which enabled rapid, sensitive and selective quantitation of creatinine directly in unprocessed urine. The mechanism of this sensor entails the creatinine-mediated aggregation of the cc-AgNPs (within 1 min) under alkaline conditions (pH 12). This is attributed to the tautomerization of creatinine to its amino anionic species at alkaline pH, which cross-link the cc-AgNPs via hydrogen bond networks with the negatively charged citrate caps. Creatinine elicited visibly-discernable color changes of the cc-AgNPs colloids in a concentration-dependent manner up to 10 μM. UV-visible spectroscopic analyses of the cc-AgNPs revealed that creatinine elicited a concentration-dependent decrease in intensity of the localized surface plasmon resonance (LSPR) band centered around 403 nm, with a concomitant increase in intensity of the red-shifted LSPR band at 670 nm. This observation denotes a creatinine-mediated increase in cc-AgNP particle size via aggregation, as confirmed by transmission electron microscopy analysis. The cc-AgNP sensor exhibited a linear correlation between the A670/A403 extinction ratio and creatinine concentration range of 0–4.2 μM in aqueous solutions (R2 = 0.996), and a low detection limit of 53.4 nM. Hence, the simplicity, short assay time, and high sensitivity and selectivity of our cc-AgNP sensor affirms its utility as a creatinine monitoring assay for low-resource, point-of-care settings. |
| Databáze: | OpenAIRE |
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