Effect of UV irradiation on colorectal cancer cells with acquired TRAIL resistance
| Autor: | Seong C. Kim, Yong J. Lee, Sung-Soo Park |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Ultraviolet Rays
Colorectal cancer Cell Apoptosis Biochemistry TNF-Related Apoptosis-Inducing Ligand Cell Line Tumor medicine Humans Cytotoxicity Molecular Biology Caspase biology Cytochrome c Cytochromes c Cell Biology medicine.disease medicine.anatomical_structure Drug Resistance Neoplasm Caspases Immunology Cancer cell biology.protein Cancer research Ultraviolet Therapy Tumor necrosis factor alpha Colorectal Neoplasms |
| Zdroj: | Journal of Cellular Biochemistry. 104:1172-1180 |
| ISSN: | 1097-4644 0730-2312 |
| Popis: | Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily. TRAIL shows strong cytotoxicity to many cancer cells but minimal cytotoxicity to most normal cells. Interestingly, our recent studies have demonstrated that pretreatment with TRAIL induces acquired resistance to TRAIL (Song et al. 2007 J Biol Chem 282: 319). Acquired TRAIL resistance develops within 1 day and gradually decays within 5 days after TRAIL treatment. In our current study, we examined whether human colorectal carcinoma CX-1 cells with acquired TRAIL resistance are resistant to UV irradiation as well. CX-1 cells were treated with 200 ng/ml TRAIL for 6 h and incubated various times (0.25–5 days) and then challenged to UV irradiation. Unexpectedly, we observed an increase in apoptosis in acquired TRAIL resistant cells after UVC as well as UVB exposure. This was due to an increase in caspase activation which was mediated through cytochrome c release. These results suggest that cells with acquired TRAIL resistance are sensitive to UV irradiation. J. Cell. Biochem. 104: 1172–1180, 2008. © 2008 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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