Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts
| Autor: | William Ditcham, Olivier Laczka, Peter L. Bergquist, Anwar Sunna, Danny K.Y. Wong, Lucy Skillman, Brenton Hamdorf |
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| Rok vydání: | 2013 |
| Předmět: |
Cryptosporidium parvum
Microbiology (medical) animal diseases Screen printed electrode Potentiometric titration Oocysts Enzyme-Linked Immunosorbent Assay Cryptosporidium Biology biology.organism_classification Microbiology Rapid detection Antibodies parasitic diseases Potentiometry Electrodes Molecular Biology |
| Zdroj: | Journal of Microbiological Methods. 95:182-185 |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/j.mimet.2013.08.014 |
| Popis: | We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5 × 10(2)Cryptosporidium oocysts per mL in 60 min. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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