Application of simultaneous determination of 3H, 14C, and 22Na by liquid scintillation counting to the measurement of cellular lon-transport
| Autor: | Yuka Miyazawa, Naoyuki Murakami, Tetsuya Konishi, Naoko Sakai |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Halobacterium salinarum
Radioisotopes Quenching Light Sodium Radioisotopes Chemistry Counting efficiency Liquid scintillation counting Biophysics Analytical chemistry Biological Transport Cell Biology Halobacterium halobium Hydrogen-Ion Concentration Tritium Biochemistry Spectral line Beta Particles Archaeobacteria Cations Scintillation Counting Carbon Radioisotopes Nuclide Molecular Biology Ion transporter |
| Zdroj: | Analytical Biochemistry. 198:194-199 |
| ISSN: | 0003-2697 |
| Popis: | A liquid scintillation counting method for simultaneous determination of three radioactive nuclides ( 3 H, 14 C, and 22 Na) of biological interest was studied. By comparing the β spectra of the three nuclides, their counting energy ranges, A, B, and C, were determined. 22 NA was set high enough to avoid any spillover counts from lower-energy nuclides. Region A for 3 H was set to maximize the counting efficiency. A good correlation between the counting efficiency for 22 Na in region C and the counting efficiency of other nuclides in all regions was obtained. Prior to 3 H and 14 C dpm calculations, the 22 Na counts spilled down in regions A and B were subtracted from the total counts in regions A and B. A simple linear equation was then used to compute 3 H and 14 C dpm. Findings show that the method presented is adaptable for highly quenched samples up to quenching indices of t sie = 100. The method is useful for studying the biological transport coupled to Na + . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|