Transgenerational effects of microcystin-LR on Daphnia magna
| Autor: | Thanh-Son Dao, Rafael Ortiz-Rodriguez, Claudia Wiegand |
|---|---|
| Přispěvatelé: | Leibniz Institute of Freshwater Ecology & Inland Fisheries, Institute for Environment and Resources, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
zooplankton
microcystin Microcystins cyanobacterial toxin Physiology Offspring Daphnia magna Microcystin-LR Microcystin 010501 environmental sciences Aquatic Science 01 natural sciences Daphnia Malate dehydrogenase Andrology 03 medical and health sciences chemistry.chemical_compound Malate Dehydrogenase Toxicity Tests Animals Biomass Molecular Biology Ecology Evolution Behavior and Systematics 030304 developmental biology 0105 earth and related environmental sciences Glutathione Transferase chemistry.chemical_classification 0303 health sciences biology L-Lactate Dehydrogenase Environmental Exposure biology.organism_classification Catalase Survival Analysis Glutathione S-transferase chemistry Biochemistry Animals Newborn 13. Climate action Insect Science transgenerational effect biology.protein Animal Science and Zoology Female Marine Toxins [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
| Zdroj: | Journal of Experimental Biology Journal of Experimental Biology, 2012, 215 (16), pp.2795-2805. ⟨10.1242/jeb.069211⟩ Journal of Experimental Biology, The Company of Biologists, 2012, 215 (16), pp.2795-2805. ⟨10.1242/jeb.069211⟩ Ortiz Rodriguez, R, Dao, S T & Wiegand, C 2012, ' Transgenerational effects of Microcystin-LR on Daphnia magna ', Journal of Experimental Biology, vol. 215, pp. 2795-2805 . |
| ISSN: | 0022-0949 1477-9145 |
| Popis: | SUMMARYAnthropogenic and climate factors increase the frequency of problematic cyanobacterial blooms in freshwater. Among other toxins, some cyanobacteria produce microcystins (MCs), which inhibit phosphatases type 1 and type 2A and provokes oxidative stress. Toxic cyanobacteria affect the growth, survival and reproduction of zooplankton, particularly those from the genus Daphnia, which have a central position in pelagic food webs. However, one possibility to ameliorate effects is to biotransform MC via glutathione S transferase (GST) to a less toxic glutathione conjugate. This process was hypothesised to underlie the ability of Daphnia to withstand MC and to explain the enhanced tolerance of the offspring from mothers exposed to toxic cyanobacteria. Thus we conducted multigenerational experiments with D. magna, exposing the parental generation to MC for 1 or 7 days and determining the enzyme-mediated tolerance to MC in their offspring by assessing the acute effect of MC on biotransformation and antioxidant and metabolism enzymes, and through 21 day chronic tests on toxicity and growth. Seven days of exposure of the parental generation to MC induced higher activity of GST and malate dehydrogenase in the offspring and enabled them to increment the catalase activity when challenged with MC, whereas 1 day of exposure of the parental generation did not. Offspring from non-exposed and 1-day-exposed mothers suffered decreased survival when exposed to MC compared with offspring from 7-day-exposed mothers; survival was correlated with the elevated activity of GST, malate dehydrogenase and catalase, suggesting maternal transfer of activation factors. However, increased survival occurred at the expense of individual growth. These results suggest that transgenerational effects are provoked by MC in D. magna, which may explain the observed acquirement of enhanced tolerance over generations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|