Frameshift mutations at two hotspots in vasopressin transcripts in post-mitotic neurons
| Autor: | F. W. Van Leeuwen, J.P.H. Burbach, M. A. F. Sonnemans, A. A. M. Van Der Kleij, D.A.P. Evans |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Vasopressin Transcription Genetic Somatic cell Vasopressins Molecular Sequence Data Hypothalamus Gene Expression Mitosis Biology medicine.disease_cause Polymerase Chain Reaction Frameshift mutation medicine Animals Amino Acid Sequence Cloning Molecular Frameshift Mutation DNA Primers Sequence Deletion Genetics Neurons Multidisciplinary Base Sequence Cell Cycle Rats Brattleboro Rats Inbred Strains Cell cycle biology.organism_classification Immunohistochemistry Brattleboro rat Rats nervous system Magnocellular cell Female Carcinogenesis hormones hormone substitutes and hormone antagonists Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 91(13) |
| ISSN: | 0027-8424 |
| Popis: | Mutations in DNA underlie carcinogenesis, inherited pathology, and aging and are generally thought to be introduced during meiosis and mitosis. Here we report that in post-mitotic neurons specific frameshift mutations occur at high frequency. These mutations were identified in vasopressin transcripts in magnocellular neurons of the homozygous Brattleboro rat and predominantly consist of a GA deletion in GAGAG motifs. Immunocytochemistry provides evidence for similar events in wild-type rats. However, the diseased state of the Brattleboro rat, resulting in a permanent activation of vasopressin neurons, enhanced the mutational rate. These data reveal hitherto unrecognized somatic mutations in nondividing neurons. |
| Databáze: | OpenAIRE |
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