Landscape of transposable elements focusing on the B chromosome of the cichlid fish astatotilapia latifasciata
| Autor: | Cesar Martins, Rafael Luiz Buogo Coan |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Transposable element Supernumerary elements medicine.medical_specialty lcsh:QH426-470 Heterochromatin Evolution Genomics Biology Genome Article cytogenetics 03 medical and health sciences Cytogenetics Cichlid evolution genomics Genetics medicine RNA-Seq Repetitive elements Extra chromosomes Genetics (clinical) Genomic organization B chromosome biology.organism_classification repetitive elements lcsh:Genetics supernumerary elements extra chromosomes 030104 developmental biology |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Genes Genes, Vol 9, Iss 6, p 269 (2018) Genes; Volume 9; Issue 6; Pages: 269 |
| Popis: | Made available in DSpace on 2018-12-11T17:37:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) B chromosomes (Bs) are supernumerary elements found in many taxonomic groups. Most B chromosomes are rich in heterochromatin and composed of abundant repetitive sequences, especially transposable elements (TEs). B origin is generally linked to the A-chromosome complement (A). The first report of a B chromosome in African cichlids was in Astatotilapia latifasciata, which can harbor 0, 1, or 2 Bs Classical cytogenetic studies found high a TE content on this B chromosome. In this study, we aimed to understand TE composition and expression in the A. latifasciata genome and its relation to the B chromosome. We used bioinformatics analysis to explore the genomic organization of TEs and their composition on the B chromosome. The bioinformatics findings were validated by fluorescent in situ hybridization (FISH) and real-time PCR (qPCR). A. latifasciata has a TE content similar to that of other cichlid fishes and several expanded elements on its B chromosome. With RNA sequencing data (RNA-seq), we showed that all major TE classes are transcribed in the brain, muscle, and male and female gonads. An evaluation of TE transcription levels between B-and B+ individuals showed that few elements are differentially expressed between these groups and that the expanded B elements are not highly transcribed. Putative silencing mechanisms may act on the B chromosome of A. latifasciata to prevent the adverse consequences of repeat transcription and mobilization in the genome. Department of Morphology Institute of Biosciences São Paulo State University (UNESP) Department of Morphology Institute of Biosciences São Paulo State University (UNESP) CNPq: 134446/2014-3 FAPESP: 2013/04533-3 FAPESP: 2014/16763-6 FAPESP: 2015/16661-1 CNPq: 305321/2015-3 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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