Transcriptional profiling of sugarcane leaves and roots under progressive osmotic stress reveals a regulated coordination of gene expression in a spatiotemporal manner
| Autor: | Jesús Alejandro Zamora-Briseño, Victor M. Gonzalez-Mendoza, Felipe Sanchez-Teyer, Miguel A. Keb-Llanes, Alejandro Pereira-Santana, Luis David Alcaraz, Jorge T. Ayala-Sumuano, Edyciel J. Alvarado-Robledo, Francisco Espadas-Gil, Luis Carlos Rodríguez-Zapata, Enrique Castaño |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Leaves Transcription Genetic Gene Expression lcsh:Medicine Plant Science Plant Roots Biochemistry Transcriptome chemistry.chemical_compound Plant Resistance to Abiotic Stress Gene expression lcsh:Science Abscisic acid Regulation of gene expression Multidisciplinary Ecology Plant Anatomy Eukaryota food and beverages Agriculture Genomics Plants Saccharum Plant Physiology Transcriptome Analysis Research Article Osmotic shock Drought tolerance Crops Biology 03 medical and health sciences Osmotic Pressure Plant-Environment Interactions Osmotic Shock DNA-binding proteins Genetics Gene Regulation Plant Defenses Grasses Gene business.industry Gene Expression Profiling Plant Ecology Ecology and Environmental Sciences lcsh:R Organisms Biology and Life Sciences Proteins Computational Biology Cell Biology Sugarcane Plant Pathology Genome Analysis Biotechnology Regulatory Proteins Gene expression profiling Plant Leaves 030104 developmental biology chemistry lcsh:Q business Crop Science Transcription Factors |
| Zdroj: | PLoS ONE, Vol 12, Iss 12, p e0189271 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Sugarcane is one of the most important crops worldwide and is a key plant for the global production of sucrose. Sugarcane cultivation is severely affected by drought stress and it is considered as the major limiting factor for their productivity. In recent years, this plant has been subjected to intensive research focused on improving its resilience against water scarcity; particularly the molecular mechanisms in response to drought stress have become an underlying issue for its improvement. To better understand water stress and the molecular mechanisms we performed a de novo transcriptomic assembly of sugarcane (var. Mex 69–290). A total of 16 libraries were sequenced in a 2x100 bp configuration on a HiSeq-Illumina platform. A total of 536 and 750 genes were differentially up-regulated along with the stress treatments for leave and root tissues respectively, while 1093 and 531 genes were differentially down-regulated in leaves and roots respectively. Gene Ontology functional analysis showed that genes related to response of water deprivation, heat, abscisic acid, and flavonoid biosynthesis were enriched during stress treatment in our study. The reliability of the observed expression patterns was confirmed by RT-qPCR. Additionally, several physiological parameters of sugarcane were significantly affected due to stress imposition. The results of this study may help identify useful target genes and provide tissue-specific data set of genes that are differentially expressed in response to osmotic stress, as well as a complete analysis of the main groups is significantly enriched under this condition. This study provides a useful benchmark for improving drought tolerance in sugarcane and other economically important grass species. |
| Databáze: | OpenAIRE |
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