Microarray Analysis on Gene Regulation by Estrogen, Progesterone and Tamoxifen in Human Endometrial Stromal Cells
| Autor: | Hai Bin Chen, Christian Lyle, David Byck, Jinping Li, Karl C. Podratz, Chun E. Ren, Xueqiong Zhu, Sean C. Dowdy, Shi Wen Jiang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Stromal cell medicine.drug_class Biology progesterone Endometrium Catalysis Article Malignant transformation Inorganic Chemistry lcsh:Chemistry Internal medicine medicine stroma estrogen Humans Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy Cells Cultured Oligonucleotide Array Sequence Analysis Regulation of gene expression uterus tamoxifen Microarray analysis techniques Organic Chemistry Estrogens General Medicine Cell cycle Computer Science Applications medicine.anatomical_structure Endocrinology lcsh:Biology (General) lcsh:QD1-999 Gene Expression Regulation Receptors Estrogen Estrogen Cancer research MCF-7 Cells Female Stromal Cells transcription Receptors Progesterone Tamoxifen medicine.drug |
| Zdroj: | International Journal of Molecular Sciences Volume 16 Issue 3 Pages 5864-5885 International Journal of Molecular Sciences, Vol 16, Iss 3, Pp 5864-5885 (2015) |
| ISSN: | 1422-0067 |
| Popis: | Epithelial stromal cells represent a major cellular component of human uterine endometrium that is subject to tight hormonal regulation. Through cell-cell contacts and/or paracrine mechanisms, stromal cells play a significant role in the malignant transformation of epithelial cells. We isolated stromal cells from normal human endometrium and investigated the morphological and transcriptional changes induced by estrogen, progesterone and tamoxifen. We demonstrated that stromal cells express appreciable levels of estrogen and progesterone receptors and undergo different morphological changes upon hormonal stimulation. Microarray analysis indicated that both estrogen and progesterone induced dramatic alterations in a variety of genes associated with cell structure, transcription, cell cycle, and signaling. However, divergent patterns of changes, and in some genes opposite effects, were observed for the two hormones. A large number of genes are identified as novel targets for hormonal regulation. These hormone-responsive genes may be involved in normal uterine function and the development of endometrial malignancies. |
| Databáze: | OpenAIRE |
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