Odorant receptors regulate the final glomerular coalescence of olfactory sensory neuron axons
| Autor: | Arie S. Mobley, Fumiaki Imamura, Diego J. Rodriguez-Gil, Charles A. Greer, Austin W. Jaspers, Dianna L. Bartel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Olfactory Nerve
Sensory Receptor Cells Neurogenesis Mitosis Kidney Receptors Odorant Olfactory Receptor Neurons Mice GAP-43 Protein Olfactory nerve Cell Movement medicine Basic Helix-Loop-Helix Transcription Factors Cell Adhesion Animals Neurons Afferent Axon In Situ Hybridization Neurons Multidisciplinary biology Axon extension Stem Cells Cell Differentiation respiratory system Biological Sciences Immunohistochemistry Olfactory Bulb Sensory neuron Axons Olfactory bulb Smell Tamoxifen medicine.anatomical_structure Electroporation nervous system Odorants biology.protein Axon guidance sense organs Olfactory epithelium Olfactory marker protein Neuroscience |
| Popis: | Odorant receptors (OR) are strongly implicated in coalescence of olfactory sensory neuron (OSN) axons and the formation of olfactory bulb (OB) glomeruli. However, when ORs are first expressed relative to basal cell division and OSN axon extension is unknown. We developed an in vivo fate-mapping strategy that enabled us to follow OSN maturation and axon extension beginning at basal cell division. In parallel, we mapped the molecular development of OSNs beginning at basal cell division, including the onset of OR expression. Our data show that ORs are first expressed around 4 d following basal cell division, 24 h after OSN axons have reached the OB. Over the next 6+ days the OSN axons navigate the OB nerve layer and ultimately coalesce in glomeruli. These data provide a previously unidentified perspective on the role of ORs in homophilic OSN axon adhesion and lead us to propose a new model dividing axon extension into two phases. Phase I is OR-independent and accounts for up to 50% of the time during which axons approach the OB and begin navigating the olfactory nerve layer. Phase II is OR-dependent and concludes as OSN axons coalesce in glomeruli. |
| Databáze: | OpenAIRE |
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