Molecular prevalence of intestinal parasites infections in children with diarrhea in Franceville, Southeast of Gabon

Autor: Jean Bernard Lekana-Douki, Nal Kennedy Ndjangangoye, Lady Charlene Kouna, Franck Mounioko, Sylvie Kwedi Nolna, Sandrine Lydie Oyegue-Liabagui, Gwladys Mirlande Lekolo
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Diarrhea
Male
0301 basic medicine
medicine.medical_specialty
030231 tropical medicine
030106 microbiology
Helminthiasis
Cryptosporidiosis
Cryptosporidium
Intestinal parasite
medicine.disease_cause
Polymerase Chain Reaction
Gastroenterology
lcsh:Infectious and parasitic diseases
Feces
03 medical and health sciences
0302 clinical medicine
Risk Factors
Internal medicine
Protozoan infection
parasitic diseases
Prevalence
medicine
Animals
Humans
Helminths
lcsh:RC109-216
Gabon
Enterocytozoon bieneusi
Intestinal Diseases
Parasitic
Dientamoeba fragilis
Protozoan Infections
biology
business.industry
Infant
biology.organism_classification
medicine.disease
Cross-Sectional Studies
Infectious Diseases
Child
Preschool
Trichuris trichiura
Female
medicine.symptom
business
Cryptosporidium hominis
Research Article
Zdroj: BMC Infectious Diseases, Vol 20, Iss 1, Pp 1-11 (2020)
BMC Infectious Diseases
ISSN: 1471-2334
Popis: Background Pediatric diarrhea caused by a range of pathogens, including intestinal parasites, is one of main causes of death among children under 5 years of age. The distribution of these parasitic infections overlaps in many environmental, socioeconomic and epidemiological settings. Their distribution and prevalence varies from region to region. In the current study, we assess the prevalence of intestinal parasites among pediatric patients with syndromic diarrheal disease living in Franceville, Gabon. Methods A cross-sectional study conducted in the Amissa Bongo Regional Hospital and Chinese-Gabonese Friendship Hospital in Franceville, between November 2016 and August 2017, enrolled a total of 100 diarrheic children between 0 and 180 months of age. Parasite detection in stool samples was performed using molecular diagnostic by PCR. Difference in means were tested by Student’s t test and ANOVA while principal component analysis was used to determine the correlation between parasite distributions and age groups. Results The overall prevalence of intestinal parasite infection was 61% (61/100). Hymenolepis sp and Cryptosporidium hominis/parvum were the most common parasites (31 and 19%, respectively), followed by Encephalitozoon intestinalis (15%), Trichuris trichiura (4%), Dientamoeba fragilis (4%), and Enterocytozoon bieneusi (2%). The polyparasitism rate was 19.7%, with 83.3% double and 16.7% triple infections. Protozoan infections (66.7%) were more prevalent than helminths infections (33.3%). Seasonal association of the circulation of intestinal parasite was statistically significant (p = 0.03). Correlations between different parasites was also observed. Conclusion The prevalence of intestinal parasitic infections is highest in diarrheic pediatric children. The prevalence of parasitic infections indicates that protozoa and helminths are the most common parasites in the Franceville environment. This study reinforces the importance of routine examination of diarrheic stool samples for the diagnostic of intestinal parasites. Further analyses are required to better understand the local epidemiology and risk factors associated with the transmission of intestinal parasites in Franceville, Gabon. Keyswords diarrhea, children, intestinal parasitic infections, molecular diagnostic, Franceville, Gabon.
Databáze: OpenAIRE
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