Regulation of lncRNA PVT1 on miR-125 in metastasis of gastric cancer cells
| Autor: | Xiaomei Zhang, Xiangxin Song, Jiatai Niu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Transwell Gastric Lymph Node Metastasis 03 medical and health sciences 0302 clinical medicine Gentamicin protection assay medicine PVT1 Cancer staging Oncogene business.industry gastric cancer digestive oral and skin physiology Cancer Articles miR-125 medicine.disease Molecular medicine digestive system diseases 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer cell Cancer research business |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2019.11195 |
| Popis: | Regulation of long non-coding RNA (lncRNA) PVT1 on miR-125 affecting the metastasis of gastric cancer cells and the mechanism were investigated. qPCR was used to detect the expression of PVT1 and miR-125 in gastric cancer and paracancerous tissues, and the relationship between PVT1 and clinicopathological parameters of gastric cancer patients. Dual luciferase reporter gene was used to detect the mutual effect between PVT1 and miR-125. The clone formation assay was used to detect changes of proliferation behavior of gastric cancer cells after inhibition of PVT1. Transwell invasion assay was used to detect the changes of invasion ability of gastric cancer cells after inhibition of PVT1. Subcutaneous tumor formation in nude mice inhibited the effect of PVT1 on the tumor size and volume of gastric cancer cells. Compared with paracancerous tissues, the expression of PVT1 and miR-125 was significantly increased in gastric cancer tissues. There were no significant differences in the expression level of PVT1 between gastric cancer patients of different genders and ages. The higher the gastric cancer staging was, the more obvious the expression level of PVT1 in the tissues of patients with gastric cancer was, and the more obvious the expression of PVT1 in the tissues of patients with gastric lymph node metastasis was. PVT1 specifically bound to the 3′UTR of miR-125, whereas the inhibition of PVT1 inhibited the ability of proliferation and invasion of gastric cancer cells. In vitro experiment of tumor formation in nude mice showed that the tumor volume and weight of the tumor-bearing mice in the si-PVT1 group were significantly reduced. PVT1 plays an important role in the occurrence and development of gastric cancer, it can regulate the proliferation and invasion of gastric cancer cells by targeting miR-125 activity. |
| Databáze: | OpenAIRE |
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