Absence of Fungal Spore Internalization by Bronchial Epithelium in Mouse Models Evidenced by a New Bioimaging Approach and Transmission Electronic Microscopy
| Autor: | Jean-Christophe Olivo-Marin, Dea Garcia-Hermoso, Fabrice de Chaumont, Charlotte Renaudat, Stéphane Bretagne, Grégory Jouvion, Fabrice Chrétien, Blandine Rammaert, Claire Szczepaniak, Françoise Dromer |
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| Přispěvatelé: | CHU Necker - Enfants Malades [AP-HP], Université Paris Diderot - Paris 7 (UPD7), Université Sorbonne Paris Cité (USPC), Université Paris Descartes - Paris 5 (UPD5), Mycologie moléculaire, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Histopathologie humaine et Modèles animaux, Institut Pasteur [Paris], Analyse d'Images biologiques, Centre Imagerie Cellulaire Santé (CICS), Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Centre Hospitalier Sainte Anne [Paris], Hôpital Saint-Louis, Université Paris Diderot - Paris 7 (UPD7)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Supported by the Prix de la Chancellerie des Universités de Paris, LegsPoix and the Institut Pasteur (Program de Recherche transversal, PTR 409).B.R. is recipient of Institut Pasteur, Ecole Polytechnique, Arts et MetiersParisTech, École supérieure de physique et de chimie industrielles de laville de Paris (ESPCI Paris Tech), Université de technologie de Compiégne(UTC), Commissariat á l’énergie atomique et aux énergies alternatives(CEA), CNRS, Labex BioPsy, and AP-HP fellowships., We thank Huot Khun for technical assistance with histology, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7) |
| Rok vydání: | 2015 |
| Předmět: |
MESH: Sordariales
Male MESH: Sequence Analysis DNA Pathology Cell Membrane Permeability Lichtheimia corymbifera MESH: Molecular Diagnostic Techniques Aspergillosis Epithelium Aspergillus fumigatus Mice MESH: Animals MESH: Cell Membrane Permeability MESH: Phagocytosis Internalization Lung [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology MESH: Treatment Outcome media_common MESH: Middle Aged biology Spores Fungal respiratory system medicine.anatomical_structure MESH: Young Adult MESH: Epithelial Cells MESH: Survival Analysis MESH: Microscopy Electron Transmission MESH: Aspergillus fumigatus medicine.medical_specialty media_common.quotation_subject Phagocytosis Pathology and Forensic Medicine MESH: Mycoses Microscopy Electron Transmission MESH: Spores Fungal medicine Animals Humans MESH: Lung MESH: Aspergillosis MESH: Mice MESH: Humans fungi Epithelial Cells medicine.disease biology.organism_classification MESH: Male respiratory tract diseases Spore MESH: Epithelium Disease Models Animal MESH: Disease Models Animal |
| Zdroj: | American Journal of Pathology American Journal of Pathology, American Society for Investigative Pathology, 2015, 185 (9), pp.2421-2430. ⟨10.1016/j.ajpath.2015.04.027⟩ American Journal of Pathology, 2015, 185 (9), pp.2421-2430. ⟨10.1016/j.ajpath.2015.04.027⟩ |
| ISSN: | 0002-9440 1525-2191 |
| DOI: | 10.1016/j.ajpath.2015.04.027 |
| Popis: | International audience; Clinical data and experimental studies suggest that bronchial epithelium could serve as a portal of entry for invasive fungal infections. We therefore analyzed the interactions between molds and the bronchial/bronchiolar epithelium at the early steps after inhalation. We developed invasive aspergillosis (Aspergillus fumigatus) and mucormycosis (Lichtheimia corymbifera) murine models that mimic the main clinical risk factors for these infections. Histopathology studies were completed with a specific computer-assisted morphometric method to quantify bronchial and alveolar spores and with transmission electron microscopy. Morphometric analysis revealed a higher number of bronchial/bronchiolar spores for A. fumigatus than L. corymbifera. The bronchial/bronchiolar spores decreased between 1 and 18 hours after inoculation for both fungi, except in corticosteroid-treated mice infected with A. fumigatus, suggesting an effect of cortisone on bronchial spore clearance. No increase in the number of spores of any species was observed over time at the basal pole of the epithelium, suggesting the lack of transepithelial crossing. Transmission electron microscopy did not show spore internalization by bronchial epithelial cells. Instead, spores were phagocytized by mononuclear cells on the apical pole of epithelial cells. Early epithelial internalization of fungal spores in vivo cannot explain the bronchial/bronchiolar epithelium invasion observed in some invasive mold infections. The bioimaging approach provides a useful means to accurately enumerate and localize the fungal spores in the pulmonary tissues. |
| Databáze: | OpenAIRE |
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