Characterization, in vitro cytotoxicity assessment, and in vivo visualization of multimodal, RITC-labeled, silica-coated magnetic nanoparticles for labeling human cord blood–derived mesenchymal stem cells
| Autor: | Jae-Berm Park, Cheol Hee Moon, Bong-Kum Choi, Jinsung Tae, Sung-Joo Kim, Junsung Kim, Jung Hee Lee, Sa Hyun Kim, Jinhyun Kim, Young Seok Kim, Jong Eun Lee, Ki Soo Park, Han Sin Lee, Jae-Won Joh |
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| Rok vydání: | 2010 |
| Předmět: |
Materials science
Biocompatibility Cell Survival Biomedical Engineering Contrast Media Pharmaceutical Science Medicine (miscellaneous) Apoptosis Bioengineering Nanotechnology Magnetics In vivo Live cell imaging Humans General Materials Science Cells Cultured Drug Carriers Staining and Labeling Rhodamines Mesenchymal stem cell Mesenchymal Stem Cells Fetal Blood Image Enhancement Silicon Dioxide Magnetic Resonance Imaging In vitro Cell biology Cord blood Nanoparticles Molecular Medicine Stem cell |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 6:263-276 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2009.07.005 |
| Popis: | Live imaging is a powerful technique that can be used to characterize the fate and location of stem cells in animal models. Here we investigated the characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, MNPs@SiO 2 (RITC). We also conducted various in vivo–uptake tests with nanoparticle-labeled human MSCs. MNPs@SiO 2 (RITC) showed photostability against ultraviolet light exposure and were nontoxic to human MSCs, based on the MTT, apoptosis, and cell cycle arrest assays. In addition, MNPs@SiO 2 (RITC) did not affect the surface phenotype or morphology of human MSCs. We also demonstrated that MNPs@SiO 2 (RITC) have stable retention properties in MSCs in vitro. Furthermore, using optical and magnetic resonance imaging, we successfully detected a visible signal from labeled human MSCs that were transplanted into NOD.CB17-Prkdc SCID (NOD-SCID) mice. These results demonstrate that MNPs@SiO 2 (RITC) are biocompatible and useful tools for human MSC labeling and bioimaging. From the Clinical Editor The characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, RITC were investigated in this study. RITC showed photostability against ultraviolet light exposure and was nontoxic to human MSCs. Using both optical and magnetic resonance imaging, successful detection of signal from labeled human MSCs transplanted into mice is demonstrated. |
| Databáze: | OpenAIRE |
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