A 2x folic acid treatment affects epigenetics and dendritic spine densities in SHSY5Y cells
| Autor: | Hunter McLeod, Daniel F. Clark, Kimberly R. Shorter, Nuri Smith, Whitnei Smith, Stewart Glenister, Nicole Rogers, Gabriel Castillo, Rahaf Al Sayed |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Dendritic spine FA Folic Acid Folic acid Cell Biophysics Biochemistry Dendritic spines MECP2 lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine Internal medicine Gene expression medicine DNMT DNA Methyltransferase lcsh:QD415-436 Epigenetics lcsh:QH301-705.5 DNA methylation biology Chemistry Histone modifications food and beverages MECP2 Methyl-CpG Binding Protein 2 ASD Autism Spectrum Disorders HDAC Histone Deacetylase Enzyme assay HMT Histone Methyltransferase 030104 developmental biology medicine.anatomical_structure Endocrinology Histone lcsh:Biology (General) 030220 oncology & carcinogenesis biology.protein HAT Histone Acetyltransferase MBD2 Methyl-CpG Binding Domain Protein 2 Research Article |
| Zdroj: | Biochemistry and Biophysics Reports, Vol 20, Iss, Pp-(2019) Biochemistry and Biophysics Reports |
| ISSN: | 2405-5808 |
| Popis: | Many diseases are now associated with aberrant epigenetics and gene expression changes. Epigenetics can be modified by factors like diet. One dietary factor, folic acid, is consumed in various forms including supplements, energy drinks, and fortified grains. It was hypothesized high levels of folic acid would affect gene expression and enzyme activity of chromatin modifying enzymes as well as dendritic spine densities in a commonly utilized neuron model, the SHSY5Y cell. Decreased MBD2 and MECP2 were discovered upon treatment of SHSY5Y cells with a 2x folic acid dose. Corresponding decreases in dendritic spines were apparent in the 2x folic acid treated cells as well. Activity of DNMTs and H3K4 HMTs was altered. Further, H3K4me1, H3K4me3, H3K9Ac, and global DNA methylation were decreased in the 2x folic acid treated cells. Further studies are warranted to determine if the effects of excess folic acid are detrimental to organismal physiology. Highlights • The 2x folic acid treatment in SHSY5Y cells induces a significant decrease in MBD2 and MECP2 mRNA expression. • The excess folic acid also causes a significant increase in dendritic spines in SHSY5Y cells. • The 2x folic acid treatment increases DNMT activity and significantly decreases H3K4 HMT activity in SHSY5Y cells. • These findings align with some of those seen in autism spectrum disorders. |
| Databáze: | OpenAIRE |
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