Cathepsin B and glutathione peroxidase show differing transcriptional responses in the grass shrimp, Palaemonetes pugio following exposure to three xenobiotics
| Autor: | Thomas W. Greig, Joseph M. Quattro, G. T. Chandler, Robert J. Griffitt |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Biology Gene Expression Regulation Enzymologic Cathepsin B Xenobiotics Toxicology Lethal Dose 50 chemistry.chemical_compound Gene expression Environmental Chemistry Animals Endosulfan Fipronil chemistry.chemical_classification Glutathione Peroxidase Decapoda Reverse Transcriptase Polymerase Chain Reaction Glutathione peroxidase Palaemonetes pugio General Chemistry biology.organism_classification Molecular biology Shrimp chemistry Pyrazoles Palaemonidae Xenobiotic Cadmium |
| Zdroj: | Environmental sciencetechnology. 40(11) |
| ISSN: | 0013-936X |
| Popis: | The common molecular biology techniques, suppressive-subtractive hybridization (SSH) and semi-quantitative real-time PCR (SQRT-PCR), were used to identify differentially expressed genes in the grass shrimp, Palaemonetes pugio following exposure to three different xenobiotics. Lab-acclimated adult male grass shrimp were exposed to empirically derived 96-hr male-specific LC50 concentrations of fipronil (FP, a phenylpyrazole GABA disrupting pesticide), endosulfan (ES, a cyclodiene GABA disrupting pesticide), or cadmium (Cd), as well as a control (CC). An SSH gene expression library was constructed from surviving shrimp from the fipronil and control exposures. Clones obtained by SSH were identified by searching against the NCBI website. A total of 42 genes were identified that were up-regulated by FP exposure, and 47 that were down-regulated. A subset of the affected genes was tested with SQRT-PCR to verify responsiveness to fipronil, as well as to endosulfan and cadmium. Two genes showed strong and significant responses to the exposures: glutathione peroxidase was significantly up-regulated by all three exposures, while Cathepsin B was strongly responsive to the two pesticides, but not to cadmium. |
| Databáze: | OpenAIRE |
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