Von Hippel–Lindau Gene Therapy: A Novel Strategy in Limiting Endothelial Cell Proliferative Activity
| Autor: | Theresa Jacob, Enrico Ascher, William Yorkovich, Anil Hingorani, Yilmaz Gunduz, Prem Seth |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Vascular Endothelial Growth Factor A
von Hippel-Lindau Disease Tumor suppressor gene Endothelial Growth Factors Transfection urologic and male genital diseases Adenoviridae chemistry.chemical_compound Humans Medicine Viability assay Aorta Cells Cultured Lymphokines Vascular Endothelial Growth Factors business.industry Gene Transfer Techniques Genetic Therapy General Medicine Molecular biology Vascular endothelial growth factor Vascular endothelial growth factor B Endothelial stem cell Vascular endothelial growth factor A chemistry Vascular endothelial growth factor C Immunology cardiovascular system Surgery Endothelium Vascular Cardiology and Cardiovascular Medicine business Cell Division |
| Zdroj: | Annals of Vascular Surgery. 15:1-6 |
| ISSN: | 0890-5096 |
| DOI: | 10.1007/s100160010011 |
| Popis: | There is evidence that loss of function of the von Hippel-Lindau (VHL) gene causes transcriptional activation of the vascular endothelial growth factor (VEGF) gene, which in turn may lead to increased proliferation of vascular endothelial cells. We hypothesized that transfer of VHL gene, a tumor suppressor gene, into vascular endothelial cells could cause loss of viability and suppression of its proliferative ability. Human aortic endothelial cells (HAEC) were grown as monolayers and transfected with varying titers of adenovirus containing the VHL cDNA (AdVHL). The negative controls used were adenovirus containing green fluorescent protein (AdGFP), vector alone (AdNull), and infection medium without virus. Adenovirus encoding p53 (Adp53) was used as positive control. Cell viability and proliferation were determined by trypan blue dye exclusion and by a tetrazolium-based colorimetric assay. All experiments were performed in triplicate. Our results showed that proliferative activity in HAEC can be blocked and viability of HAEC reduced by adenovirus-mediated gene transfer of VHL gene. This is the first time that VHL gene has been effectively transferred to HAEC. VHL gene transfer into the vascular endothelium may have potential in limiting proliferative processes, including intimal hyperplasia. |
| Databáze: | OpenAIRE |
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