Autophagy is essential for optimal translocation of iron to seeds in Arabidopsis
| Autor: | Céline Masclaux-Daubresse, Sébastien Thomine, Mathieu Pottier, Jean Dumont |
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| Přispěvatelé: | Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Approches intégratives du Transport Ionique (MINION), Département Biologie Cellulaire (BioCell), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, Département Biochimie, Biophysique et Biologie Structurale (B3S), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE20-0019,ISISTOR,Amélioration du contenu en fer de la graine(2016) |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine metal leaf senescence Physiology Iron [SDV]Life Sciences [q-bio] education Population Mutant Arabidopsis Chromosomal translocation Plant Science 01 natural sciences iron loading 03 medical and health sciences Nutrient iron recycling Autophagy micronutrient Arabidopsis thaliana Micronutrients health care economics and organizations 57Fe 2. Zero hunger Manganese education.field_of_study biology Chemistry food and beverages Biological Transport 15. Life on land Micronutrient biology.organism_classification Research Papers Cell biology Zinc remobilization premature senescence 030104 developmental biology Seeds Growth and Development fe-57 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany Journal of Experimental Botany, 2019, 70 (3), pp.859--869. ⟨10.1093/jxb/ery388⟩ Journal of Experimental Botany, Oxford University Press (OUP), 2019, 70 (3), pp.859--869. ⟨10.1093/jxb/ery388⟩ |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/ery388 |
| Popis: | Autophagy is an essential recycling mechanism making micronutrients available in vegetative organs for subsequent reallocation to seeds Micronutrient deficiencies affect a large part of the world’s population. These deficiencies are mostly due to the consumption of grains with insufficient content of iron (Fe) or zinc (Zn). Both de novo uptake by roots and recycling from leaves may provide seeds with nutrients. Autophagy, which is a conserved mechanism for nutrient recycling in eukaryotes, was shown to be involved in nitrogen remobilization to seeds. Here, we have investigated the role of this mechanism in micronutrient translocation to seeds. We found that Arabidopsis thaliana plants impaired in autophagy display defects in nutrient remobilization to seeds. In the atg5-1 mutant, which is completely defective in autophagy, the efficiency of Fe translocation from vegetative organs to seeds was severely decreased even when Fe was provided during seed formation. Combining atg5-1 with the sid2 mutation that counteracts premature senescence associated with autophagy deficiency and using 57Fe pulse labeling, we propose a two-step mechanism in which Fe taken up de novo during seed formation is first accumulated in vegetative organs and subsequently remobilized to seeds. Finally, we show that translocation of Zn and manganese (Mn) to seeds is also dependent on autophagy. Fine-tuning autophagy during seed formation opens up new possibilities to improve micronutrient remobilization to seeds. |
| Databáze: | OpenAIRE |
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