Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
| Autor: | Carolina Gay, Silvana Maruñak, Pamela Teibler, Laura Leiva, Ofelia Acosta |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Myotoxin Venom Hemorrhage Pharmacology Toxicology medicine.disease_cause complex mixtures HEMORRHAGE Mice Muscular Diseases Neutralization Tests Crotalid Venoms medicine Animals Bothrops Envenomation Muscle Skeletal Polyclonal specific antibodies biology Toxin Antivenins Ciencias Veterinarias BALTERGIN SVMPs Bothrops alternatus biology.organism_classification CIENCIAS AGRÍCOLAS Snake venom Polyclonal antibodies MYOTOXICITY Immunoglobulin G Immunology biology.protein Metalloproteases |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 63 |
| ISSN: | 1879-3150 |
| Popis: | Myotoxicity, one of the most relevant local manifestations in envenomation by Bothrops genus, may result from a direct action of myotoxins or be due to an indirect vascular degeneration and ischemia. Baltergin, a snake venom metalloproteinase (SVMP), isolated from Bothrops alternatus venom has been used to obtain monospecific IgG, in order to determine the relative role of toxin in myotoxicity induced by whole venom. Bothrops diporus venom, another medical relevant genus of the northeastern region of Argentina, was also studied. Anti-baltergin IgG was able to neutralize completely the hemorrhagic activity of B. alternatus venom at an antibodies:venom ratio of 30:1 (w:w). However, mice injected with B. diporus venom showed a small spot remaining even at the highest ratio of IgG:venom assayed (50:1; w:w). Specific antibodies were efficient to neutralize the myotoxicity of B. alternatus venom at ratio 30:1 (w:w) but did not neutralize the same effects in B. diporus venom. Anti-baltergin polyclonal antibodies were useful tools for revealing the central role of SVMPs in the development of myotoxicity of B. alternatus venom, as well as, helping to suggest indirectly presence of potent myotoxic phospholipases A2 (PLA2s) in B. diporus venom. Fil: Gay, Claudia Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina Fil: Maruñak, Silvana. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina Fil: Teibler, Pamela. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina Fil: Leiva, Laura. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina Fil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina |
| Databáze: | OpenAIRE |
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