Annexin A3 is necessary for parallel artery-vein alignment in the mouse retina
| Autor: | Kelsey Carter, Stryder M. Meadows, Avery Blanks, Ondine Cleaver, Katie Huang, Nehal R. Patel, Angela M. Crist |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Cell Xenopus Biology Retina Article Veins 03 medical and health sciences Mice 0302 clinical medicine Annexin Cell Movement Cell Line Tumor Conditional gene knockout medicine Animals Annexin A3 Cell Proliferation medicine.disease biology.organism_classification Cell biology 030104 developmental biology medicine.anatomical_structure cardiovascular system Branch retinal vein occlusion Female 030217 neurology & neurosurgery Developmental Biology Artery |
| Zdroj: | Dev Dyn |
| ISSN: | 1097-0177 |
| Popis: | BACKGROUND: Annexin A3 (Anxa3) is a member of the calcium-regulated, cell membrane-binding family of annexin proteins. We previously confirmed that Anxa3 is expressed in the endothelial lineage in vertebrates and that loss of anxa3 in Xenopus laevis leads to embryonic blood vessel defects. However, the biological function of Anxa3 in mammals is completely unknown. In order to investigate Anxa3 vascular function in mammals, we generated an endothelial cell-specific Anxa3 conditional knockout mouse model (Anxa3(f/f);Tie2-Cre). RESULTS: Anxa3(f/f);Tie2-Cre mice are born at Mendelian ratios and display morphologically normal blood vessels during development. However, loss of Anxa3 leads to artery-vein (AV) misalignment characterized by atypical AV crossovers in the postnatal and adult retina. CONCLUSIONS: Anxa3 is not essential for embryonic blood vessel formation but is required for proper parallel AV alignment in the murine retina. AV crossovers associated with Anxa3(f/f);Tie2-Cre mice are similar to AV intersections observed in patients with branch retinal vein occlusion (BRVO), although we did not observe occluded vessels. This new Anxa3 mouse model may provide a basis for understanding AV crossover formation associated with BRVO. |
| Databáze: | OpenAIRE |
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