Age‐Related Absorption of the Human Lens in the Near‐Ultraviolet Range
| Autor: | Béla Hopp, Antal Nógrádi, Lajos Kolozsvári, Ferenc Rárosi, Viktor Pajer |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
0301 basic medicine 030103 biophysics Materials science Adolescent Ultraviolet Rays Analytical chemistry medicine.disease_cause Biochemistry law.invention Absorbance Young Adult 03 medical and health sciences 0302 clinical medicine law Age related Lens Crystalline medicine Humans Physical and Theoretical Chemistry Near ultraviolet Child Absorption (electromagnetic radiation) Aged Range (particle radiation) Age Factors General Medicine Middle Aged Lens (optics) Wavelength 030220 oncology & carcinogenesis Spectrophotometry Ultraviolet Ultraviolet |
| Zdroj: | Photochemistry and Photobiology. 96:826-833 |
| ISSN: | 1751-1097 0031-8655 |
| DOI: | 10.1111/php.13199 |
| Popis: | The purpose of the present study was to determine the age dependence of the ultraviolet (UV) absorption of the different parts of the human crystalline lens. Cryostat sections of human cadaveric lenses (60 μm) were cut. The UV absorbance of nine samples, derived from different parts of the lens, was determined using a Shimadzu scanning spectrophotometer. The absorbance of the anterior and posterior lens capsules was measured separately. The absorption coefficients were calculated from the measured absorbance and values taken at 280 as well as at 360 nm were compared statistically. ANCOVA analysis of the values taken at 280 and at 360 nm wavelengths shows that correlation between the absorption coefficients and age can be found only in the case of the posterior layers. These results suggest a differential age-dependent increase of the UV absorption of the posterior layers compared to the anterior ones and can be related to the differential protein expression in the anterior and posterior parts. Posterior crystalline lens capsules have higher absorption coefficients than the anterior ones regardless of age. |
| Databáze: | OpenAIRE |
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