Comparative analysis of sphingomyelin synthase 1 gene expression at the transcriptional and translational levels in human tissues
| Autor: | Ivan B. Filippenkov, Lyudmila V. Dergunova, Svetlana A. Limborska, Olga Yu. Sudarkina, Ilya Brodsky |
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| Rok vydání: | 2015 |
| Předmět: |
Gene isoform
Transcription Genetic Clinical Biochemistry Transferases (Other Substituted Phosphate Groups) Nerve Tissue Proteins Kidney RNA interference Sphingomyelin synthase SNAP23 Gene expression Protein biosynthesis Humans Molecular Biology HSPA9 Binding Sites biology Membrane Proteins Cell Biology General Medicine Isoenzymes Biochemistry Organ Specificity Enzyme Induction Protein Biosynthesis biology.protein RNA Interference Enzyme Repression Sphingomyelin |
| Zdroj: | Molecular and Cellular Biochemistry. 406:91-99 |
| ISSN: | 1573-4919 0300-8177 |
| Popis: | Sphingomyelin synthase 1 (SMS1) catalyses the biosynthesis of sphingomyelin in eukaryotic cells. We have previously determined the structure of the SGMS1 gene encoding this enzyme and a number of its alternative transcripts. Here, we describe a study of the expression of the full-length SMS1 protein and the sum of the alternative transcripts encoding this protein in human tissues. The SMS1 protein and mRNA levels in tissues differed significantly and were not correlated, implying the active post-transcriptional regulation of SMS1 protein expression. The putative truncated isoforms of the SMS1 protein, which are encoded by a number of alternative transcripts, were not detected by immunoblotting and thus may be absent or present in only small amounts. |
| Databáze: | OpenAIRE |
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