Revealing links between gut microbiome and its fungal community in Type 2 Diabetes Mellitus among Emirati subjects: A pilot study

Autor: Karine Clément, Mohammad Tahseen Al Bataineh, Nihar Ranjan Dash, Pierre Bel Lassen, Bayan Hassan Banimfreg, Aml Mohamed Nada, Eugeni Belda
Přispěvatelé: University of Sharjah (UoS), Nutrition et obésités: approches systémiques (nutriomics) (UMR-S 1269 INSERM - Sorbonne Université), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Service de nutrition [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), American University of Sharjah, Integromics [Paris], Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], This work was supported by Research Institute of Medical and Health Sciences at University of Sharjah grant P1701090226 (M.A.B), Boehringer Ingelheim grant 2016-17 (N.R.D), EU litmus grant (K.C), Le Ducq foundation (K.C. and P.B.L), and JPI-HDHL MICRODIET consortium grant (K.C and P.B.L)., Service de Nutrition [CHU Pitié-Salpétrière], Institut E3M [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Bodescot, Myriam, Nutrition et obésités: approches systémiques (UMR-S 1269) (Nutriomics), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière] (IHU ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière] (IHU ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
endocrine system diseases
Prevotella
Pilot Projects
Overweight
Polymerase Chain Reaction
0302 clinical medicine
RNA
Ribosomal
16S
Bacteroides
2. Zero hunger
Genetics
education.field_of_study
Multidisciplinary
biology
Fungal genetics
Endocrine system and metabolic diseases
Middle Aged
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Medicine
Female
Enterotype
medicine.symptom
Microbial genetics
Adult
Science
Population
United Arab Emirates
Microbial communities
030209 endocrinology & metabolism
Article
Young Adult
03 medical and health sciences
medicine
Humans
Microbiome
Author Correction
education
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Aged
nutritional and metabolic diseases
Type 2 Diabetes Mellitus
RNA
Fungal
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
biology.organism_classification
[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Gastrointestinal Microbiome
030104 developmental biology
Diabetes Mellitus
Type 2
Mycobiome
Zdroj: Scientific Reports
Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.9624. ⟨10.1038/s41598-020-66598-2⟩
Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
Scientific Reports, 2020, 10 (1), pp.9624. ⟨10.1038/s41598-020-66598-2⟩
ISSN: 2045-2322
DOI: 10.1038/s41598-020-66598-2
Popis: Type 2 diabetes mellitus (T2DM) drastically affects the population of Middle East countries with an ever-increasing number of overweight and obese individuals. The precise links between T2DM and gut microbiome composition remain elusive in these populations. Here, we performed 16 S rRNA and ITS2- gene based microbial profiling of 50 stool samples from Emirati adults with or without T2DM. The four major enterotypes initially described in westernized cohorts were retrieved in this Emirati population. T2DM and non-T2DM healthy controls had different microbiome compositions, with an enrichment in Prevotella enterotype in non-T2DM controls whereas T2DM individuals had a higher proportion of the dysbiotic Bacteroides 2 enterotype. No significant differences in microbial diversity were observed in T2DM individuals after controlling for cofounding factors, contrasting with reports from westernized cohorts. Interestingly, fungal diversity was significantly decreased in Bacteroides 2 enterotype. Functional profiling from 16 S rRNA gene data showed marked differences between T2DM and non-T2DM controls, with an enrichment in amino acid degradation and LPS-related modules in T2DM individuals, whereas non-T2DM controls had increased abundance of carbohydrate degradation modules in concordance with enterotype composition. These differences provide an insight into gut microbiome composition in Emirati population and its potential role in the development of diabetes mellitus.
Databáze: OpenAIRE
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