Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated
| Autor: | Manja Litjens, G. Snijders, Lot D. de Witte, Elly M. Hol, Alba Fernández-Andreu, Woutje M. Berdowski, Marjolein A. M. Sneeboer, Hans C. van Mierlo, Amber Berdenis van Berlekom, René S. Kahn |
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| Přispěvatelé: | Netherlands Institute for Neuroscience (NIN) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Bipolar Disorder Thalamus Molecular neuroscience Research Support Article lcsh:RC321-571 Pathogenesis 03 medical and health sciences Superior temporal gyrus Cellular and Molecular Neuroscience 0302 clinical medicine Immune system medicine Journal Article Humans Non-U.S. Gov't lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Biological Psychiatry Aged Aged 80 and over Cerebral Cortex Microglia business.industry Research Support Non-U.S. Gov't Middle Aged Medial frontal gyrus Psychiatry and Mental health 030104 developmental biology medicine.anatomical_structure nervous system Immunology Biomarker (medicine) Female Autopsy business Biomarkers 030217 neurology & neurosurgery Ex vivo |
| Zdroj: | Translational Psychiatry, 9(1). Nature Publishing Group Translational Psychiatry, Vol 9, Iss 1, Pp 1-10 (2019) Translational Psychiatry |
| ISSN: | 2158-3188 |
| Popis: | Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies have addressed the involvement of microglia in BD so far and a more detailed immune profiling of microglial activation is lacking. Here, we applied a multi-level approach to determine the activation state of microglia in BD post-mortem brain tissue. We did not find differences in microglial density, and mRNA expression of microglial markers in the medial frontal gyrus (MFG) of patients with BD. Furthermore, we performed in-depth characterization of human primary microglia isolated from fresh brain tissue of the MFG, superior temporal gyrus (STG), and thalamus (THA). Similarly, these ex vivo isolated microglia did not show elevated expression of inflammatory markers. Finally, challenging the isolated microglia with LPS did not result in an increased immune response in patients with BD compared to controls. In conclusion, our study shows that microglia in post-mortem brain tissue of patients with BD are not immune activated. |
| Databáze: | OpenAIRE |
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