Production of selectable marker gene-free Cavendish banana (Musa spp.) using a steroid-inducible recombinase platform
| Autor: | Anthony Brinin, Jean-Yves Paul, Benjamin Dugdale, Jennifer Kleidon, James L. Dale, Robert M. Harding |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Agrobacterium Genetic Vectors Green Fluorescent Proteins Plant disease resistance Biology 01 natural sciences Banana Recombinases 03 medical and health sciences Gene Expression Regulation Plant Genetics Recombinase Gene Selectable marker Disease Resistance Reporter gene Original Paper Marker-free food and beverages Musa biology.organism_classification Plants Genetically Modified Cavendish banana Transformation (genetics) 030104 developmental biology Cavendish Animal Science and Zoology Steroids Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | Transgenic Research |
| ISSN: | 1573-9368 |
| Popis: | Genetic improvement of commercially accepted banana cultivars is strongly reliant on the ability to introduce genes that encode important agro-traits such as disease resistance. In most cases this can only be achieved using a transgenic approach. Public and regulatory acceptance of these events would greatly increase with “clean” single copy integration events free of the selectable marker gene and extraneous vector backbone. This would also allow for the successive addition of new genes and traits as they become available. In this study, we used the pMarker Free 1 (pMF1) vector containing the green fluorescent protein (gfp) reporter gene to assess the effectiveness of steroid-inducible recombination and positive/negative dual selection to regenerate transgenic Cavendish banana plants that were potentially free of the selectable marker gene. By examining the interaction of two different Agrobacterium strains with two different cultivars of Cavendish banana, namely Williams and Grand Naine, we describe a transformation and regeneration strategy that successfully produced marker-free, single transgene copy, gfp-expressing events. The system will provide a useful means of serially improving banana into the future. |
| Databáze: | OpenAIRE |
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