Mutagenicity, metabolism and DNA adduct formation of 6-nitrochrysene in Salmonella typhimurium
| Autor: | Stephen S. Hecht, Karam El-Bayoumy, Gong Huey Shiue, Robert H. Heflich, K. Barry Delcios, Ralph P. Walker |
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| Rok vydání: | 1989 |
| Předmět: |
Male
Salmonella typhimurium Health Toxicology and Mutagenesis Metabolite In Vitro Techniques Toxicology Chrysenes Ames test chemistry.chemical_compound In vivo DNA adduct Genetics Animals Incubation Genetics (clinical) Biotransformation biology Mutagenicity Tests Metabolism DNA Phenanthrenes biology.organism_classification Enterobacteriaceae Rats Inbred F344 Rats Biochemistry chemistry Liver Carcinogens Mutagens |
| Zdroj: | Mutagenesis. 4(3) |
| ISSN: | 0267-8357 |
| Popis: | The mutagenic activities of 6-nitrochrysene (6-NC) and its previously identified metabolites were evaluated in Salmonella typhimurium TA100 and TA98 in the presence and absence of metabolic activation by 9000 g supernatant from the livers of rats treated with Aroclor. 6-Aminochrysene (6-AC) and trans-1,2-dihydro-1,2-dihydroxy-6-aminochrysene (1,2-DHD-6-AC) were the most active mutagens in TA100 upon metabolic activation. 6-NC and 6-AC were the most active mutagens in TA100 in the absence of metabolic activation. Upon metabolic activation, 6-AC was the most active in TA98; the other compounds were weak or inactive depending on the conditions of the assay. In the absence of metabolic activation, the mutagenic activities of 6-NC and its metabolites in TA98 were comparable to those observed in TA100. The major metabolite formed upon incubation of [3H]6-NC with S.typhimurium TA100 and 9000 g supernatant from the livers of Aroclor-induced rats was identified as trans-1,2-dihydro-1,2-dihydroxy-6-nitrochrysene (1,2-DHD-6-NC); trans-9,10-dihydro-9,10-dihydroxy-6-nitrochrysene and 1,2-dihydroxy-6-nitrochrysene were also identified. The major DNA adduct formed in TA100 under these conditions was chromatographically identical to that previously detected in vivo in the liver and lungs of newborn mice treated with 6-NC, as well as to that obtained upon incubation of 1,2-DHD-6-AC with calf thymus DNA in the presence of rat liver microsomes. The DNA adducts derived from 6-NC in S.typhimurium TA100 without activation were identical to those adducts previously identified after incubation of 6-hydroxylaminochrysene with calf thymus DNA.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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