Aged mice exhibit in vivo defective peripheral clonal deletion of Db/H-Y reactive CD8+ T cells
| Autor: | Huang-Ge Zhang, Hui-Chen Hsu, Horst Bluethmann, Jian Shi, John D. Mountz, Di Liu, Tong Zhou, Ping Ar Yang |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Aging
Fas Ligand Protein CD3 Complex CD3 H-Y Antigen Apoptosis Spleen Mice SCID CD8-Positive T-Lymphocytes Kidney Clonal deletion Major Histocompatibility Complex Interferon-gamma Mice Immune system medicine Animals Cytotoxic T cell Histocompatibility Antigen H-2D Lung Membrane Glycoproteins biology H-2 Antigens T lymphocyte Adoptive Transfer Molecular biology Mice Inbred C57BL Hyaluronan Receptors Phenotype medicine.anatomical_structure Liver biology.protein Female Lymph Nodes Antibody CD8 Developmental Biology |
| Zdroj: | Mechanisms of Ageing and Development. 122:305-326 |
| ISSN: | 0047-6374 |
| DOI: | 10.1016/s0047-6374(00)00247-5 |
| Popis: | We previously reported that T cells from aged mice were resistant to activation-induced cell death (AICD) in vitro. To determine whether the presence of AICD-resistant T cells is associated with defects in age-related peripheral clonal deletion in vivo, congenic male SCID mice were reconstituted with T cells from aged or young female D(b)/H-Y TCR (Tg71) transgenic mice. Compared with recipients of young cells, the recipients of T cells from aged mice exhibited a 3-fold increase in the percentage of autoreactive CD8(+) H-Y antigen-reactive T cells as defined by the clonotypic antibody, M33. There were significantly increased sera levels of interferon-gamma, a significantly decreased expression of FasL by M33(+)CD8(+) T cells, and significantly decreased apoptosis by DNA fragmentation staining of the spleen of mice reconstituted with T cells from aged mice compared to those from young mice. By day 21, the recipients of T cells from aged mice but not young mice, exhibited infiltration of CD3(+) cells into the non-lymphoid organs. These results indicate that there is defective peripheral deletion of the self-reactive T cells derived from aged female Tg71 mice, and that failure to delete these cells is associated with the defective T-cell clonal deletion in the recipient mice. |
| Databáze: | OpenAIRE |
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