Evaluation of the developmental potential of metaphase I oocytes from stimulated intracytoplasmic sperm injection cycles
| Autor: | Huai-Liang Feng, Shuiying Ma, Keliang Wu, Hui-Jun Yang, Yuan Li, Wanxia Zhong, Li Che, Mei Li, Zi-Jiang Chen |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Time Factors medicine.medical_treatment Embryonic Development Reproductive technology Biology Oogenesis Intracytoplasmic sperm injection Andrology Endocrinology Pregnancy Genetics medicine Humans Sperm Injections Intracytoplasmic Molecular Biology Metaphase Fertilisation Chi-Square Distribution Embryo culture Anatomy Embryo Transfer In vitro maturation Reproductive Medicine embryonic structures Oocytes Female Animal Science and Zoology Folliculogenesis Spermatogenesis Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 23:433 |
| ISSN: | 1031-3613 |
| Popis: | The objective of the present study was to evaluate the developmental potential and clinical application value of metaphase I (MI) oocytes obtained from stimulated intracytoplasmic sperm injection (ICSI) cycles. ICSI was performed on MI oocytes immediately after denudation (Group A), or on in vitro-matured (IVM) oocytes following culture; oocytes in culture were further divided into two groups, being cultured for either 3–5 h (Group B) or 24–28 h (Group C). Metaphase II oocytes from the same cycle(s) isolated for ICSI served as the control group (Group D). The rates of normal fertilisation, cleavage and high-quality embryos were compared among the four groups. High-quality embryos were transferred whenever possible, and pregnancy rates were evaluated. Results showed that normal fertilisation rates for Groups B, C and D were significantly higher than that of Group A (68.6%, 57.8%, 74.5% and 30.1%, respectively; P |
| Databáze: | OpenAIRE |
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