Integrated economic and experimental framework for screening of primary recovery technologies for high cell density CHO cultures
| Autor: | Suzanne S. Farid, Daria Popova, Adam Stonier, David Pain, Nigel J. Titchener-Hooker |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Computer science Cell Survival Lab scale Nanotechnology Context (language use) Cell Count Centrifugation CHO Cells 01 natural sciences Applied Microbiology and Biotechnology Cross-flow filtration law.invention Cell Line Mammalian cell culture 03 medical and health sciences Cricetulus law Batch Cell Culture Techniques 010608 biotechnology Mammalian cell Cricetinae Process economics Animals High cell density Bioseparation Process engineering Filtration Research Articles Cell Proliferation business.industry High cell General Medicine Tangential flow filtration Primary recovery 030104 developmental biology Molecular Medicine business Research Article Biotechnology |
| Zdroj: | Biotechnology Journal |
| ISSN: | 1860-7314 1860-6768 |
| Popis: | Increases in mammalian cell culture titres and densities have placed significant demands on primary recovery operation performance. This article presents a methodology which aims to screen rapidly and evaluate primary recovery technologies for their scope for technically feasible and cost‐effective operation in the context of high cell density mammalian cell cultures. It was applied to assess the performance of current (centrifugation and depth filtration options) and alternative (tangential flow filtration (TFF)) primary recovery strategies. Cell culture test materials (CCTM) were generated to simulate the most demanding cell culture conditions selected as a screening challenge for the technologies. The performance of these technology options was assessed using lab scale and ultra scale‐down (USD) mimics requiring 25–110mL volumes for centrifugation and depth filtration and TFF screening experiments respectively. A centrifugation and depth filtration combination as well as both of the alternative technologies met the performance selection criteria. A detailed process economics evaluation was carried out at three scales of manufacturing (2,000L, 10,000L, 20,000L), where alternative primary recovery options were shown to potentially provide a more cost‐effective primary recovery process in the future. This assessment process and the study results can aid technology selection to identify the most effective option for a specific scenario. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text