SIEVE ELEMENT-LINING CHAPERONE1 restricts aphid feeding on arabidopsis during heat stress
| Autor: | Jacqueline Busscher-Lange, Karen J. Kloth, G.L. Wiegers, Marcel Dicke, Willem Kruijer, Maarten A. Jongsma, Rhonda C. Meyer, Benedicte R. Albrectsen, Harro J. Bouwmeester, Gonda Buijs |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mutant Plant Science 01 natural sciences Wiskundige en Statistische Methoden - Biometris 03 medical and health sciences Arabidopsis Botany Arabidopsis thaliana Life Science BIOS Plant Development Systems Laboratorium voor Plantenfysiologie Laboratory of Entomology Mathematical and Statistical Methods - Biometris Aphid biology fungi food and beverages Cell Biology biology.organism_classification PE&RC Laboratorium voor Entomologie Cell biology 030104 developmental biology Biometris Inflorescence Ultrastructure BIOS Applied Metabolic Systems Phloem Silique EPS Laboratory of Plant Physiology 010606 plant biology & botany |
| Zdroj: | Plant Cell 29 (2017) 10 Plant Cell, 29(10), 2450-2464 |
| ISSN: | 1040-4651 2450-2464 |
| Popis: | The role of phloem proteins in plant resistance to aphids is still largely elusive. By genome-wide association mapping of aphid behavior on 350 natural Arabidopsis thaliana accessions, we identified the small heat shock-like SIEVE ELEMENT-LINING CHAPERONE1 (SLI1). Detailed behavioral studies on near-isogenic and knockout lines showed that SLI1 impairs phloem feeding. Depending on the haplotype, aphids displayed a different duration of salivation in the phloem. On sli1 mutants, aphids prolonged their feeding sessions and ingested phloem at a higher rate than on wild-type plants. The largest phenotypic effects were observed at 26°C, when SLI1 expression is upregulated. At this moderately high temperature, sli1 mutants suffered from retarded elongation of the inflorescence and impaired silique development. Fluorescent reporter fusions showed that SLI1 is confined to the margins of sieve elements where it lines the parietal layer and colocalizes in spherical bodies around mitochondria. This localization pattern is reminiscent of the clamp-like structures observed in previous ultrastructural studies of the phloem and shows that the parietal phloem layer plays an important role in plant resistance to aphids and heat stress. |
| Databáze: | OpenAIRE |
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