Alterations in Intestinal Microbiota of Children With Celiac Disease at the Time of Diagnosis and on a Gluten-free Diet
| Autor: | Konstantinos Gerasimidis, Antonia Karanikolou, Tracey Cardigan, Mary Mackinder, Olga Biskou, Clare M. Clark, Hazel Duncan, Richard Hansen, Konstantina Zafeiropoulou, Paraic McGrogan, Julie Russell, Ben Nichols, David Wands, Christine A. Edwards, Richard K. Russell, Eleni Rizou, Umer Zeeshan Ijaz, Elaine Buchanan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty OTU UCD untreated celiac disease Gastroenterology Pathogenesis Food group 03 medical and health sciences 0302 clinical medicine Internal medicine medicine GFD gluten-free diet OTU operational taxonomic unit Ingestion HC healthy control subjects Microbiome GIP gluten immunogenic peptide Feces Original Research chemistry.chemical_classification Pediatric Hepatology business.industry Short-chain Fatty Acids GI gastrointestinal Gluten 030104 developmental biology chemistry CD celiac disease SCFA short-chain fatty acid TCD treated celiac disease 030211 gastroenterology & hepatology Gluten free Clinical—Alimentary Tract Gastrointestinal function business BCFA branch-chain fatty acid |
| Zdroj: | Gastroenterology |
| ISSN: | 1528-0012 0016-5085 |
| Popis: | Background And Aims It is not clear whether alterations in the intestinal microbiota of children with celiac disease (CD) cause the disease or are a result of disease and/or its treatment with a gluten-free diet (GFD). Methods We obtained 167 fecal samples from 141 children (20 with new-onset CD, 45 treated with a GFD, 57 healthy children, and 19 unaffected siblings of children with CD) in Glasgow, Scotland. Samples were analyzed by 16S ribosomal RNA sequencing, and diet-related metabolites were measured by gas chromatography. We obtained fecal samples from 13 children with new-onset CD after 6 and 12 months on a GFD. Relationships between microbiota with diet composition, gastrointestinal function, and biomarkers of GFD compliance were explored. Results Microbiota α diversity did not differ among groups. Microbial dysbiosis was not observed in children with new-onset CD. In contrast, 2.8% (Bray-Curtis dissimilarity index, P = .025) and 2.5% (UniFrac distances, P = .027) of the variation in microbiota composition could be explained by the GFD. Between 3% and 5% of all taxa differed among all group comparisons. Eleven distinctive operational taxonomic units composed a microbe signature specific to CD with high diagnostic probability. Most operational taxonomic units that differed between patients on a GFD with new-onset CD vs healthy children were associated with nutrient and food group intake (from 75% to 94%) and with biomarkers of gluten ingestion. Fecal levels of butyrate and ammonia decreased during the GFD. Conclusions Although several alterations in the intestinal microbiota of children with established CD appear to be effects of a GFD, specific bacteria were found to be distinct biomarkers of CD. Studies are needed to determine whether these bacteria contribute to pathogenesis of CD. Graphical abstract |
| Databáze: | OpenAIRE |
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