Long-term cultured skeletal muscle-derived neural precursor cells and their neurogenic potentials
| Autor: | Ji Youl Lee, Soyeon Kim, Jung Sik Park, Byung Sun Yoo, Young Seok Baek, Sung Ho Ghil, Sung Hak Kang |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Time Factors Neurogenesis Blotting Western Cell Biology Cell therapy Mice Cell transplantation Internal medicine Precursor cell medicine Animals Muscle Skeletal Cells Cultured Neurons Mice Inbred ICR Reverse Transcriptase Polymerase Chain Reaction Stem Cells General Neuroscience Transdifferentiation Skeletal muscle Cell Differentiation Immunohistochemistry Neuronal disease Cell biology Endocrinology medicine.anatomical_structure Bromodeoxyuridine Stem cell |
| Zdroj: | NeuroReport. 20:1109-1114 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/wnr.0b013e32832e9b21 |
| Popis: | For cell-based therapy, it is necessary to obtain sufficient cell quantities for cell transplantation to the diseased or injured site. However, a given tissue has only a limited number of stem cells, making it necessary to expand stem cell source through long-term culture. In this study, we evaluated whether our recently described skeletal muscle-derived neural precursor (SMNP) cells can be cultured long-term without alteration of their neural precursor characteristics. Our results showed that SMNP cells can be cultured over approximately 16 months, but their growth rate and neurogenic potential gradually decrease in a culture time-dependent manner. Importantly, approximately 120-day cultured SMNP cells retain their self-renewal ability, neural precursor characteristics, and high neurogenic potential. These results suggest that SMNP cells may be an appropriate cell source for regenerative or reparative therapies against neuronal disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|