Longitudinal Near-Infrared Imaging of Myelination
| Autor: | Chunying Wu, Changning Wang, Robert H. Miller, Daniela C. Popescu, Wendy B. Macklin, Junqing Zhu, Yanming Wang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Nervous system
Fluorescence-lifetime imaging microscopy Time Factors Mice Transgenic Corpus callosum Permeability Article Corpus Callosum Cuprizone Mice Mice Neurologic Mutants In vivo medicine Animals Benzothiazoles Longitudinal Studies Remyelination Myelin Proteolipid Protein Myelin Sheath Analysis of Variance Spectroscopy Near-Infrared biology General Neuroscience Multiple sclerosis Myelin Basic Protein Carbocyanines medicine.disease Myelin basic protein Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure biology.protein Proto-Oncogene Proteins c-akt Neuroscience Preclinical imaging Demyelinating Diseases Protein Binding |
| Zdroj: | The Journal of Neuroscience. 31:2382-2390 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | Myelination is one of the fundamental biological processes in the development of vertebrate nervous system. Disturbance of myelination is found to be associated with progression in many neurological diseases such as multiple sclerosis. Tremendous efforts have been made to develop novel therapeutic agents that prevent demyelination and/or promote remyelination. These efforts need to be accompanied by the development of imaging tools that permit direct quantification of myelinationin vivo. In this work, we describe a novel near-infrared fluorescence imaging technique that is capable of direct quantification of myelinationin vivo. This technique is developed based on a near-infrared fluorescent probe, 3,3′-diethylthiatricarbocyanine iodide (DBT) that readily enters the brain and specifically binds to myelinated fibers.In vivoimaging studies were first conducted in two animal models of hypermyelination and hypomyelination followed by longitudinal studies in the cuprizone-induced demyelination/remyelination mouse model. Quantitative analysis suggests that DBT is a sensitive and specific imaging probe of myelination, which complements other current myelin-imaging modalities and is of low cost. |
| Databáze: | OpenAIRE |
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