Possible Mechanisms of Resistance tocis-Diamminedichloroplatinum (II) of Human Ovarian Cancer Cells
| Autor: | Yoshihiro Kikuchi, Munenori Miyauchi, Yoshio Tenjin, Tsunekazu Kita, Michio Sugita, Ichiro Nagata, I. Iwano |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
inorganic chemicals
Cancer Research medicine.medical_specialty Cell Population Cystadenocarcinoma Drug Resistance Mice Nude Ovary In Vitro Techniques Biology Article Mice chemistry.chemical_compound Internal medicine medicine Animals Humans education neoplasms Incubation Cells Cultured Cross-resistance Ovarian Neoplasms education.field_of_study Cis diamminedichloroplatinum ii Dose-Response Relationship Drug DNA Flow Cytometry Molecular biology female genital diseases and pregnancy complications Carboplatin Endocrinology medicine.anatomical_structure Oncology chemistry Cell culture Female Cisplatin |
| Zdroj: | Japanese Journal of Cancer Research. 81:701-706 |
| ISSN: | 0910-5050 |
| Popis: | The mechanism of cis-diamminedichloroplatinum (II) (CDDP) resistance was examined by using a CDDP-resistant (KFr) cell line established by continuous exposure of KF cells (derived from serous cystadenocarcinoma of the ovary) to escalating doses of CDDP. When KFr cells were incubated with 66.7 microM CDDP, the uptake of CDDP was significantly inhibited and the cellular content in the KFr cells was about a half of that in KF cells after incubation for 4 h. When the KF or KFr cells were incubated for 4 h with 100 microM CDDP, the release pattern of CDDP from KFr cells was similar to that from KF cells. In addition, the DNA histogram of both KF and KFr cells revealed that KF cells seemed to contain two clones of cell population and the KFr cells may have been selected by exposure to CDDP. At 3 h after intraperitoneal administration of 0.5 mg of CDDP per mouse to nude mice with KF or KFr tumor, the CDDP content in the KFr tumor was significantly lower than that in the KF tumor. In contrast, at 6 or 9 h after CDDP administration the CDDP content in the KFr tumor was significantly higher than that in the KF tumor. Furthermore, the KFr cells had cross-resistance to various CDDP analogues including carboplatin. It was shown that cellular uptakes of two CDDP analogues into KFr cells were significantly lower than those into KF cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text