Mucosa-associated microbiota dysbiosis in colitis associated cancer
| Autor: | Giuseppina Liguori, Massimo Pierluigi Di Simone, Carlo Calabrese, Philippe Langella, Thomas W. Hoffmann, Massimo Campieri, Giovanni Brandi, Gilberto Poggioli, Mathias L. Richard, Bruno Lamas, Grégory Da Costa, Harry Sokol |
|---|---|
| Přispěvatelé: | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Departement Hopital Universitaire, Partenaires INRAE, Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Endocrinologie & Toxicologie de la Barrière Intestinale (ToxAlim-ENTeRisk), ToxAlim (ToxAlim), Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université Pierre et Marie Curie - Paris 6 (UPMC), Service de Gastroentérologie et nutrition [CHU Saint-Antoine], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Xeda International S.A, Richard, Ml, Liguori, G, Lamas, B, Brandi, G, da Costa, G, Hoffmann, Tw, Di Simone MP, Calabrese, C, Poggioli, G, Langella, P, Campieri, M, Sokol, H. |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male [SDV]Life Sciences [q-bio] Gut flora Inflammatory bowel disease Cohort Studies Intestinal mucosa Crohn Disease RNA Ribosomal 16S mucosa-associated microbiota Intestinal Mucosa Cancer Aged 80 and over biology Gastroenterology Biodiversity dysbiosis Middle Aged Colitis 3. Good health Infectious Diseases Female Colorectal Neoplasms fungal microbiota Microbiology (medical) Adult Microbiology digestive system 03 medical and health sciences DNA Ribosomal Spacer medicine Humans Aged Bacteria Ruminococcus dysbiosi Fungi biology.organism_classification medicine.disease Inflammatory Bowel Diseases Gastrointestinal Microbiome stomatognathic diseases 030104 developmental biology Fusobacterium Immunology Research Paper/Report Colitis Ulcerative Dysbiosis |
| Zdroj: | Gut microbes Gut microbes, Taylor & Francis, 2018, 9 (2), pp.131-142. ⟨10.1080/19490976.2017.1379637⟩ |
| ISSN: | 1949-0976 1949-0984 |
| Popis: | International audience; Gut microbiota dysbiosis has been associated with inflammatory bowel diseases (IBD). In colorectal cancer, the gut microbiota has also been recognized as potentially involved in aggravating or favoring the tumor development. However, very little is known on the structure and role of the microbiota in colitis associated cancer (CAC), an important complication of IBD in human. Here we analyzed the bacterial and fungal composition of the mucosa associated microbiota of patients suffering CAC, sporadic cancer (SC) and of healthy subjects (HS) by barcode sequences analysis on the following cohort: 7 CAC patients, 10 SC patients and 10 HS using 16S (MiSeq) and ITS2 (pyrosequencing) sequencing, for bacteria and fungi respectively. Mucosa-associated bacterial microbiota in CAC was significantly different from the ones in SC or in HS, while the fungal showed no differences. Comparison between mucosa-associated microbiota on the tumor site or in normal mucosa near the tumor showed very similar patterns. The global mucosa-associated bacterial microbiota in cancer patients was characterized by a restriction in biodiversity but no change for the fungal community. Compared to SC, CAC was characterized by an increase of Enterobacteriacae family and Sphingomonas genus and a decrease of Fusobacterium and Ruminococcus genus. Our study confirms the alteration of the mucosa-associated bacterial microbiota in IBD and SC. Although the cohort is limited in number, this is the first evidence of the existence of an altered bacterial microbiota in CAC clearly different from the one in SC patients. |
| Databáze: | OpenAIRE |
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