Optical biosensing of markers of mucosal inflammation
| Autor: | Bill T. Ameredes, William J. Calhoun, Allan R. Brasier, Asanka S. Yapa, Obdulia Covarrubias-Zambrano, Madumali Kalubowilage, Christopher T. Culbertson, Hongwang Wang, Yingxin Zhao, Deryl L. Troyer, Massoud Motamedi, Aruni P. Malalasekera, Bing Tian, Stefan H. Bossmann |
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| Rok vydání: | 2021 |
| Předmět: |
Lung Diseases
Chemokine medicine.medical_treatment Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Inflammation Article Mice Medicine Animals General Materials Science Exhaled breath condensate Liquid biopsy COPD biology medicine.diagnostic_test business.industry respiratory system medicine.disease Asthma respiratory tract diseases Bronchoalveolar lavage Cytokine Breath Tests Neutrophil elastase Immunology biology.protein Molecular Medicine medicine.symptom business Biomarkers |
| Zdroj: | Nanomedicine |
| ISSN: | 1549-9642 |
| Popis: | We report the design and adaptation of iron/iron oxide nanoparticle-based optical nanobiosensors for enzymes or cytokine/chemokines that are established biomarkers of lung diseases. These biomarkers comprise ADAM33, granzyme B, MMP-8, neutrophil elastase, arginase, chemokine (C-C motif) ligand 20 and interleukin-6. The synthesis of nanobiosensors for these seven biomarkers, their calibration with commercially available enzymes and cytokines/chemokines, as well as their validation using bronchoalveolar lavage (BAL) obtained from a mouse model of TLR3-mediated inflammation are discussed here. Exhaled Breath Condensate (EBC) is a minimally invasive approach for sampling airway fluid in the diagnosis and management of various lung diseases in humans (e.g., asthma, COPD and viral infections). We report the proof-of-concept of using human EBC in conjunction with nanobiosensors for diagnosis/monitoring airway inflammation. These findings suggest that, with nanosensor technology, human EBC can be utilized as a liquid biopsy to monitor inflammation/remodeling in lung disease. |
| Databáze: | OpenAIRE |
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