Polyethylene glycol-modified pokeweed mitogen (PWM) as a potential non-immunogenic stimulator of lymphokine-activated killer cells
| Autor: | Shin-ichiro Takai, Ayako Matsushima, Misao Hiroto, Yuji Inada, Yoh Kodera, Hiroyuki Nishimura, Kimoto Y, Tomoo Ueno, Katsukiyo Sakurai |
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| Rok vydání: | 1996 |
| Předmět: |
Cytotoxicity
Immunologic Materials science Cell Survival Biomedical Engineering Biophysics Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Bioengineering Cell Line Polyethylene Glycols Biomaterials Mice chemistry.chemical_compound Immune system Tumor Cells Cultured Animals Humans Killer Cells Lymphokine-Activated Cytotoxicity Lymphokine-activated killer cell biology Immunogenicity Pokeweed mitogen Molecular biology Recombinant Proteins In vitro Pokeweed Mitogens chemistry Biochemistry Antibody Formation Colonic Neoplasms biology.protein Interleukin-2 Indicators and Reagents Antibody Ethylene glycol |
| Zdroj: | Journal of Biomaterials Science, Polymer Edition. 7:753-758 |
| ISSN: | 1568-5624 0920-5063 |
| Popis: | Among the various plant lectins, pokeweed mitoge (PWM) is most effective in enhancing the cytotoxicity of human lymphokine-activated killer (LAK) cells. However, the use of PWM in adoptive immunotherapy has been limited due to the strong immune response against the protein of plant origin. Amino groups in PWM was modified with 2,4-bis[O-methoxypoly(ethylene glycol)]-6-chioro-s-triazine, activated PEG2, to form PEG-PWM conjugates. Its immunoreactivity towards anti-PWM antibodies was reduced by increasing the degree of modification of amino groups in PWM. PEG-PWM, in which 54% of amino groups in PWM was modified with activated PEG2, had a nearly complete reduction of immunoreactivity. Intraperitoneal administration of PEG-PWM to mice did not produce substantial levels of anti-PWM antibodies. Nevertheless, PEG-PWM retained the ability to induce the maximum levels of cytotoxicity of human LAK cells in vitro. |
| Databáze: | OpenAIRE |
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