Comparative study of transgenic Brachypodium distachyon expressing sucrose:fructan 6-fructosyltransferases from wheat and timothy grass with different enzymatic properties
| Autor: | Midori Yoshida, Kazuhiro Tase, Akira Kawakami, Yasuharu Sanada, Ken-ichi Tamura, Toshihiko Yamada |
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| Rok vydání: | 2014 |
| Předmět: |
Sucrose
Freezing tolerance DNA Complementary Acclimatization Carbohydrates Plant Science Genetically modified crops Gene Expression Regulation Enzymologic Degree of polymerization Fructan Phleum Gene Expression Regulation Plant Freezing Botany Genetics Cold acclimation Biomass Triticeae Triticum Plant Proteins Timothy-grass biology food and beverages Plants Genetically Modified biology.organism_classification Fructans Plant Leaves Phenotype Hexosyltransferases Wheat Brachypodium Fructosyltransferase Brachypodium distachyon Timothy |
| Zdroj: | Planta. 239:783-792 |
| ISSN: | 1432-2048 0032-0935 |
| Popis: | Fructans can act as cryoprotectants and contribute to freezing tolerance in plant species, such as in members of the grass subfamily Pooideae that includes Triticeae species and forage grasses. To elucidate the relationship of freezing tolerance, carbohydrate composition and degree of polymerization (DP) of fructans, we generated transgenic plants in the model grass species Brachypodium distachyon that expressed cDNAs for sucrose:fructan 6-fructosyltransferases (6-SFTs) with different enzymatic properties: one cDNA encoded PpFT1 from timothy grass (Phleum pratense), an enzyme that produces high-DP levans; a second cDNA encoded wft1 from wheat (Triticum aestivum), an enzyme that produces low-DP levans. Transgenic lines expressing PpFT1 and wft1 showed retarded growth; this effect was particularly notable in the PpFT1 transgenic lines. When grown at 22 A degrees C, both types of transgenic line showed little or no accumulation of fructans. However, after a cold treatment, wft1 transgenic plants accumulated fructans with DP = 3-40, whereas PpFT1 transgenic plants accumulated fructans with higher DPs (20 to the separation limit). The different compositions of the accumulated fructans in the two types of transgenic line were correlated with the differences in the enzymatic properties of the overexpressed 6-SFTs. Transgenic lines expressing PpFT1 accumulated greater amounts of mono- and disaccharides than wild type and wft1 expressing lines. Examination of leaf blades showed that after cold acclimation, PpFT1 overexpression increased tolerance to freezing; by contrast, the freezing tolerance of the wft1 expressing lines was the same as that of wild type plants. These results provide new insights into the relationship of the composition of water-soluble carbohydrates and the DP of fructans to freezing tolerance in plants. |
| Databáze: | OpenAIRE |
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