Accelerating the rate of disassembly of karyopherin.cargo complexes
| Autor: | Michael Rexach, Brook Mykytka, Daniel Gilchrist |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Nucleocytoplasmic Transport Proteins
Saccharomyces cerevisiae Proteins Stereochemistry Allosteric regulation Active Transport Cell Nucleus Saccharomyces cerevisiae Karyopherins environment and public health Biochemistry Dissociation (chemistry) Fungal Proteins Animals Nuclear pore Molecular Biology Karyopherin Monomeric GTP-Binding Proteins chemistry.chemical_classification Nucleoplasm Chemistry Nuclear Proteins Cell Biology beta Karyopherins Nuclear Pore Complex Proteins Biophysics Nucleoporin Nuclear transport Nuclear localization sequence |
| Zdroj: | The Journal of biological chemistry. 277(20) |
| ISSN: | 0021-9258 |
| Popis: | Transport of macromolecules across the nuclear pore complex (NPC) occurs in seconds and involves assembly of a karyopherin.cargo complex and docking to the NPC, translocation of the complex across the NPC via interaction with nucleoporins (Nups), and dissociation of the complex in the nucleoplasm. To identify rate-limiting steps in the Kap95p.Kap60p-mediated nuclear import pathway of Saccharomyces cerevisiae, we reconstituted key intermediate complexes and measured their rates of dissociation and affinities of interaction. We found that a nuclear localization signal-containing protein (NLS-cargo) dissociates slowly from Kap60p monomers and Kap60p.Kap95p heterodimers with half-lives (t(12)) of 7 and 73 min, respectively; that Kap60p and Kap60p.NLS-cargo complexes dissociate slowly from Kap95p (t(12) = 36 and 73 min, respectively); and that Kap95p.Kap60p.NLS-cargo complexes and Kap95p.Kap60p heterodimers dissociate rapidly from the nucleoporin Nup1p (t(12)or = 21 s) and other Nups. A search for factors that accelerate disassembly of the long-lived intermediates revealed that Nup1p and Nup2p accelerate 16- and 19-fold the rate of dissociation of NLS-cargo from Kap60p.Kap95p heterodimers; that Gsp1p-GTP acceleratesor = 447-fold the rate of dissociation of Kap60p.NLS-cargo from Kap95p; and that Nup2p and the Cse1p.Gsp1p-GTP complex independently accelerateor = 22- andor = 39-fold the rate of dissociation of NLS-cargo from Kap60p. We suggest that Nup1p, Nup2p, Cse1p, and Gsp1p accelerate disassembly of Kap95p.Kap60p.NLS-cargo complexes by triggering allosteric mechanisms within Kaps that cause rapid release of binding partners. In that way, Nup1p, Nup2p, Cse1p, and Gsp1p may function as karyopherin release factors (or KaRFs) in the nuclear basket structure of the S. cerevisiae NPC. |
| Databáze: | OpenAIRE |
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