Complexes of thrombin with secreted platelet proteins
| Autor: | Thomas C. Detwiler, Joseph J. Miller, Paul C. Browne |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Blood Platelets
HEPES Lysis Complex formation Thrombin Biophysics Prostacyclin Blood Proteins Cell Biology Epoprostenol Biochemistry chemistry.chemical_compound chemistry medicine Humans Electrophoresis Polyacrylamide Gel Platelet Platelet activation Thrombospondins Molecular Biology Calcimycin Glycoproteins medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 151:9-15 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(88)90552-9 |
| Popis: | A labeled 77-kDa complex formed when 125I-thrombin was added to platelet suspensions or to the supernatant solution of ionophore-activated platelets. Prostacyclin inhibited complex formation with whole platelets but not with the supernatant solution of ionophore-activated platelets. This is evidence that the complex formed with a factor secreted from activated platelets. Smaller complexes of 70 and 58 kDa formed between labeled thrombin and lysed platelets. The 77-kDa complex was necessary for the formation of a thrombin-thrombospondin complex. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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