Effect of growth regulators on microspore embryogenesis in coconut anthers

Autor: Deepthi Yakandawala, Jean-Luc Verdeil, Valérie Hocher, L. K. Weerakoon, P. I. P. Perera
Rok vydání: 2008
Předmět:
Zdroj: Plant Cell, Tissue and Organ Culture
ISSN: 1573-5044
0167-6857
DOI: 10.1007/s11240-008-9473-y
Popis: The effect of growth regulators on induction of androgenesis in coconut was investigated using seven different growth regulators at various concentrations and combinations. Three auxins (1-naphthalene acetic acid-NAA, indoleacetic acid-IAA, picloram) and three cytokinins (2-isopentyl adenine-2-iP, kinetin, zeatin) were tested either alone or in combination with 2,4-dichlorophenoxyacetic acid (2,4-D), using modified Eeuwens Y3 liquid medium as the basal medium. Among the tested auxins, 100 [mu]M NAA in combination with 100 [mu]M 2,4-D enhanced the production of calli/embryos (123) whereas IAA and picloram showed negative and detrimental effects, respectively, for androgenesis induction over 100 lM 2,4- D alone. Kinetin and 2-iP enhanced the production of calli/ embryos when 100 [mu]M 2,4-D was present in the culture medium. Both cytokinins at 10 [mu]M yielded the highest frequencies of embryos (113 and 93, respectively) whereas zeatin (1 or 2.5 [mu]M) had no impact on microspore embryogenesis. When calli/embryos (produced from different treatments in different experiments) were subcultured in somatic embryo induction medium (Y3 medium containing 66 [mu]M 2,4-D), followed by maturation medium (Y3 medium without growth regulators) and germination medium (Y3 medium containing 5 [mu]M-6-benzyladenine- BA and 0.35 [mu]M gibberellic acid-GA3), plantlets were regenerated at low frequencies (in most treatments ranging from 0% to 7%).
Databáze: OpenAIRE
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