CRISPR Typing and Subtyping for Improved Laboratory Surveillance of Salmonella Infections
| Autor: | Catherine Lim, Sylvie Issenhuth-Jeanjean, Véronique Guibert, Ghislaine Guigon, Jian Zhang, François-Xavier Weill, Simon Le Hello, Christophe Sola, Saïana de Romans, Sylvain Brisse, Martine Guibourdenche, Mark Achtman, Laetitia Fabre, Marie Accou-Demartin, Virginie Passet, Chrystelle Roux, Laure Diancourt |
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| Přispěvatelé: | Bactéries pathogènes entériques (BPE), Institut Pasteur [Paris] (IP), Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), Department of Microbiology and Environmental Research Institute (Cork, Ireland), University College Cork (UCC), This work was supported by the Institut Pasteur (permanent support for Research and Programme Transversal de Recherche 354) and by the Institut de Veille Sanitaire. The MLST data are publicly available from http://mlst.ucc.ie, which is currently funded by a grant from the Science Foundation of Ireland (05/FE1/B882)., Institut Pasteur [Paris] |
| Rok vydání: | 2012 |
| Předmět: |
Salmonella typhimurium
Bacterial Diseases Serotype Salmonella Salmonellosis Applied Microbiology lcsh:Medicine medicine.disease_cause law.invention law Databases Genetic CRISPR lcsh:Science MESH: Databases Genetic Polymerase chain reaction Genetics 0303 health sciences Multidisciplinary biology Subtyping Bacterial Pathogens 3. Good health Infectious Diseases MESH: Internet [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Medical Microbiology Salmonella enterica Population Surveillance Salmonella Infections Medicine Public Health MESH: Inverted Repeat Sequences Research Article Biotechnology Salmonella bongori Microbiology MESH: Population Surveillance QH301 03 medical and health sciences MESH: Polymorphism Genetic medicine Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Typing Serotyping Biology QH426 030304 developmental biology Internet Polymorphism Genetic MESH: Humans MESH: Salmonella Infections 030306 microbiology Inverted Repeat Sequences lcsh:R MESH: Serotyping Bacteriology MESH: Salmonella typhimurium biology.organism_classification lcsh:Q |
| Zdroj: | PLoS ONE PLoS ONE, 2012, 7 (5), pp.e36995. ⟨10.1371/journal.pone.0036995⟩ PLoS ONE, Vol 7, Iss 5, p e36995 (2012) PLoS ONE, Public Library of Science, 2012, 7 (5), pp.e36995. ⟨10.1371/journal.pone.0036995⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0036995 |
| Popis: | International audience; Laboratory surveillance systems for salmonellosis should ideally be based on the rapid serotyping and subtyping of isolates. However, current typing methods are limited in both speed and precision. Using 783 strains and isolates belonging to 130 serotypes, we show here that a new family of DNA repeats named CRISPR (clustered regularly interspaced short palindromic repeats) is highly polymorphic in Salmonella. We found that CRISPR polymorphism was strongly correlated with both serotype and multilocus sequence type. Furthermore, spacer microevolution discriminated between subtypes within prevalent serotypes, making it possible to carry out typing and subtyping in a single step. We developed a high-throughput subtyping assay for the most prevalent serotype, Typhimurium. An open web-accessible database was set up, providing a serotype/spacer dictionary and an international tool for strain tracking based on this innovative, powerful typing and subtyping tool. |
| Databáze: | OpenAIRE |
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