Fluorescence Studies on Glyceraldehyde- 3-phosphate Dehydrogenase from Bovine Heart Muscle
| Autor: | Ewa Seweryn, Bogdan Zieliński, Izabela Berdowska, Ireneusz Ceremuga, Teresa Banaś |
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| Rok vydání: | 2001 |
| Předmět: |
Pyruvate dehydrogenase kinase
biology Chemiosmosis Myocardium Glyceraldehyde-3-Phosphate Dehydrogenases Dehydrogenase Photophosphorylation Papillary Muscles Chromatography Ion Exchange General Biochemistry Genetics and Molecular Biology Enzyme assay Kinetics Adenosine Triphosphate Spectrometry Fluorescence Biochemistry biology.protein Animals Cattle Glycolysis Enzyme inducer Glyceraldehyde 3-phosphate dehydrogenase |
| Zdroj: | Zeitschrift für Naturforschung C. 56:1166-1168 |
| ISSN: | 1865-7125 0939-5075 |
| DOI: | 10.1515/znc-2001-11-1240 |
| Popis: | Glyceraldehyde-3-phosphate Dehydrogenase, ATP, Flu orescence Glyceraldehyde-3-phosphate dehydrogenase is a glycolytic enzyme that catalyses conversion of glyceralde-hyde-3-phosphate to 1,3-diphosphoglycerate. ATP has been found to have an inhibitory effect on this enzyme. To establish the interaction between the enzyme and ATP, a fluorescence technique was used. Fluorescence quenching in the presence of ATP suggests cooperative binding of ATP to the enzyme (the Hill obtained coefficient equals 2.78). The interaction between glyceralde-hyde-3-phosphate dehydrogenase and ATP may control not only glycolysis but other activities of this enzyme, such as binding to the cytoskeleton. |
| Databáze: | OpenAIRE |
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