The cellular retinol-binding protein genes are duplicated and differentially transcribed in the developing and adult zebrafish (Danio rerio)
| Autor: | Jonathan M. Wright, Eileen M. Denovan-Wright, Qian Sun, Rong-Zong Liu, Bernard Thisse, Christine Thisse |
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| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Male
animal structures Transcription Genetic MESH: Rats Danio MESH: Zebrafish Proteins Genome 03 medical and health sciences Mice Gene Duplication Gene duplication MESH: Gene Expression Regulation Developmental Genetics Animals Humans MESH: Animals MESH: Zebrafish Molecular Biology Zebrafish Gene Peptide sequence MESH: Mice Ecology Evolution Behavior and Systematics MESH: Organ Specificity 030304 developmental biology Regulation of gene expression 0303 health sciences MESH: Humans biology MESH: Transcription Genetic 030302 biochemistry & molecular biology fungi MESH: Gene Duplication Gene Expression Regulation Developmental Retinol-Binding Proteins Cellular [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Zebrafish Proteins biology.organism_classification MESH: Retinol-Binding Proteins MESH: Male Rats Retinol-Binding Proteins Organ Specificity embryonic structures Female RBP1 MESH: Female |
| Zdroj: | Molecular Biology and Evolution Molecular Biology and Evolution, Oxford University Press (OUP), 2005, 22 (3), pp.469-77. ⟨10.1093/molbev/msi030⟩ |
| ISSN: | 0737-4038 1537-1719 |
| DOI: | 10.1093/molbev/msi030⟩ |
| Popis: | International audience; There are single copies of the genes encoding the cellular retinol-binding protein type I and II (CRBPI and CRBPII) in the human and rodent genomes. We have identified duplicate genes for both CRBPI and CRBPII in the zebrafish (Danio rerio) genome (rbp1b and rbp2b). The zebrafish rbp1b and rbp2b have conserved gene structures, amino acid sequence similarities, gene phylogenies, and syntenic relationships with their mammalian orthologs and zebrafish paralogs, rbp1a and rbp2a. Like the mammalian genes for CRBPI and CRBPII, the zebrafish rbp1b and rbp2b genes are closely linked on a single linkage group. Comparative analysis suggests that the duplicate genes of rbp1 and rbp2 in the zebrafish genome may have arisen by chromosomal or whole-genome duplication. During embryonic development, rbp1b transcripts were detected in the gall bladder of 5-day postfertilization (5 dpf) larvae. The rbp2b mRNA was abundant in the developing liver through 48 hours postfertilization (48 hpf) to 5 dpf. Using reverse transcription-polymerase chain reaction (RT-PCR), rbp1b transcripts were detected in the ovary, and rbp2b mRNA was observed predominantly in the adult liver. Tissue section in situ hybridization and emulsion autoradiography localized rbp1b mRNA to primary oocytes within the zebrafish ovary. The differential mRNA distribution patterns of the rbp1a, rbp1b, rbp2a, and rbp2b genes in the developing and adult zebrafish suggest that shuffling of subfunctions among duplicate copies of paralogous genes may be a mechanism for the retention of duplicated genes in vertebrates. |
| Databáze: | OpenAIRE |
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