Interleukin (IL)-4 production by human T cells: differential regulation of IL-4vs. IL-2 production
| Autor: | I. Rensink, Tineke van der Pouw-Kraan, Cees van Kooten, Lucien A. Aarden |
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| Rok vydání: | 1992 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Interleukin 2 medicine.medical_specialty CD3 Complex T-Lymphocytes medicine.medical_treatment Immunology Population CD2 Antigens Receptors Antigen T-Cell Stimulation Biology chemistry.chemical_compound Internal medicine Cyclic AMP medicine Humans Immunology and Allergy Interferon gamma Receptors Immunologic education Cells Cultured Protein Kinase C Interleukin 4 education.field_of_study Interleukin Up-Regulation Endocrinology Cytokine chemistry Phorbol Interleukin-2 Tetradecanoylphorbol Acetate Interleukin-4 medicine.drug |
| Zdroj: | European Journal of Immunology. 22:1237-1241 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830220519 |
| Popis: | We have examined the regulation of interleukin (IL)-4 production by human peripheral blood T cells. Production of IL-4 was shown to be regulated differently from IL-2 and interferon(IFN)-gamma production. Stimulation of peripheral blood lymphocytes with anti-CD3, anti-CD2, anti-CD28, Phorbol 12-myristate 13-acetate (PMA) or IL-2 as a single stimulant did not induce IL-4 production. However, combinations of anti-CD2 with either anti-CD28 or IL-2 resulted in IL-4 production, peaking at days 3-4. Stimulation with anti-CD3 instead of anti-CD2 gave similar results, but was less potent. After days 3-4, IL-4 levels decreased, most likely due to consumption of IL-4. PMA profoundly affected cytokine production, it enhanced IL-2 production by at least tenfold, whereas, in the same cell population, IL-4 production was almost completely inhibited. This was observed at the protein as well as at the mRNA level. In contrast, agents that increase intracellular cAMP levels inhibited IL-2 production but left IL-4 production unaffected. IFN-gamma production behaved similar to IL-2 production but the effects were less outspoken. |
| Databáze: | OpenAIRE |
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