Empedobacter stercoris sp. nov., isolated from an input sample of a biogas plant
| Autor: | Stefanie P. Glaeser, Jan Golke, Peter Kämpfer, Thorsten Schauss, Hans-Jürgen Busse |
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| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial Spermidine Swine Molecular Sequence Data Microbiology Phylogenetics Empedobacter brevis Germany RNA Ribosomal 16S Animals Ecology Evolution Behavior and Systematics Phylogeny Base Composition Strain (chemistry) biology Phylogenetic tree Phosphatidylethanolamines Fatty Acids Bacteroidetes Vitamin K 2 General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Flavobacteriaceae Bacterial Typing Techniques Manure Biochemistry Biofuels Cattle Bacteria |
| Zdroj: | International journal of systematic and evolutionary microbiology. 65(10) |
| ISSN: | 1466-5034 |
| Popis: | Two Gram-stain-negative, rod-shaped bacteria, strains 990B6_12ER2AT and 994B6_12ER2A, were isolated during microbiological analysis of a mixed manure sample which was used as input material for a German biogas plant. Phylogenetic identification based on nearly full-length 16S rRNA gene sequences placed the isolates into the family Flavobacteriaceae within the phylum Bacteroidetes. Strains 990B6_12ER2AT and 994B6_12ER2A shared identical 16S rRNA gene sequences and showed highest 16S rRNA gene sequence similarity to the type strains of Empedobacter falsenii (97.3 %) and Empedobacter brevis (96.8 %). The major cellular fatty acids of strains 990B6_12ER2AT and 994B6_12ER2A were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH. The polyamine pattern contained predominantly sym-homospermidine and the quinone system was menaquinone MK-6. Major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified polar lipid not containing an amino residue, a phosphate residue or a sugar moiety. In addition, moderate to minor amounts of several unidentified lipids were detected. The DNA G+C content was 31.7 and 29.0 mol%, for strains 990B6_12ER2AT and 994B6_12ER2A, respectively. On the basis of phylogenetic, chemotaxonomic and physiological analysis we propose a novel species of the genus Empedobacter, Empedobacter stercoris sp. nov. (type strain 990B6_12ER2AT = CIP 110833T = LMG 28501T). |
| Databáze: | OpenAIRE |
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