Diagnostic utilities of a fully automated molecular test for toxigenic Clostridium difficile
| Autor: | Megumi Annaka, Makoto Nakamura, Katsunori Yanagihara, Takashi Inamatsu, Ai Tanaka, Yasutomo Itakura, Yoshishige Masuda, Norihiko Akamatsu, Hideaki Kato, Hiroko Tateno, Sayuri Morita, Takeshi Tomaru, Junichi Matsuda, Yoshitomo Morinaga |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Bacterial Toxins 030106 microbiology Clostridium difficile toxin A Enterotoxin Biology Microbiology law.invention Enterotoxins Feces 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Glutamate Dehydrogenase Antigen Limit of Detection law Humans Pharmacology (medical) 030212 general & internal medicine Polymerase chain reaction Aged Aged 80 and over Clostridioides difficile Glutamate dehydrogenase Middle Aged Clostridium difficile Infectious Diseases Molecular Diagnostic Techniques Fully automated Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Clostridium Infections Female |
| Zdroj: | Journal of Infection and Chemotherapy. 24:88-91 |
| ISSN: | 1341-321X |
| Popis: | Laboratory underdiagnosis of toxigenic Clostridium difficile can lead to inappropriate management of C. difficile infection (CDI). A fully automated molecular test (FAMT), BD MAX, and enzyme immunoassays for C. difficile glutamate dehydrogenase (GDH) and for toxin A/B antigen test were evaluated using clinical specimens. Laboratory analysis of 231 fecal specimens from patients suspected with CDI, indicated that the sensitivity (Sn), specificity (Sp), positive predictive value (PPV), and negative predictive value (NPV) of FAMT was 98.1%, 98.9%, 96.3%, and 99.4%, while that of toxin A/B antigen was 52.8%, 100.0%, 100.0%, and 87.7%, respectively, compared to toxigenic culture. Sn, Sp, PPV, and NPV of GDH test compared to toxigenic culture was 92.5%, 94.4%, 83.1%, and 97.7%, respectively. FAMT can support the accurate laboratory diagnosis of toxigenic C. difficile and be an effective tool for appropriate treatment of CDI. |
| Databáze: | OpenAIRE |
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